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目的:研究丹参轮作期间土壤各类微生物数量和细菌生理群落结构的变化特点。方法:采用培养法计算土壤微生物菌群结构,氯仿熏蒸提取法测定土壤微生物量磷,数据采用SPSS软件分析。结果:细菌和放线菌的数量随着轮作年限的增加而增加,真菌则降低,土壤微生物量磷降低;生理细菌类生物多样性指数呈增加趋势。结论:丹参栽培中轮作的微生物机制是土壤各类微生物数量和生理类群细菌群落结构的调整,重新建立土壤生态系统平衡过程;栽培丹参后土壤微生物群落自然恢复间隔年限至少为2年,与传统的种植经验吻合。
Objective: To study the characteristics of soil microbial community and bacterial community structure during the rotation of Salvia miltiorrhiza. Methods: The microbial community structure of soil was calculated by culture method. The soil microbial biomass phosphorus was determined by chloroform fumigation extraction method. The data were analyzed by SPSS software. Results: The number of bacteria and actinomycetes increased with the increase of rotation years, but the fungi decreased and the soil microbial biomass phosphorus decreased. The index of physiological bacterial biodiversity increased. Conclusion: The microbial mechanism of rotation during the cultivation of Salvia miltiorrhiza is the adjustment of the quantity of microorganisms and the bacterial community structure of physiological groups, and the re-establishment of soil ecosystem balance process. The natural recovery interval of soil microbial community after Salvia miltiorrhiza is cultivated is at least 2 years, Planting experience is consistent.