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为探讨亚甲蓝对体外培养的人大肠癌细胞的抗肿瘤作用 ,采用直接杀伤实验、细胞生长抑制实验和细胞集落形成能力实验方法研究了亚甲蓝对体外培养的人大肠癌细胞系Lovo的作用 ,同时用透射电镜技术观察了该药对癌细胞的损伤部位 .结果显示 13 2 5 μmol/L亚甲蓝作用2 4h可杀死 79 4%的大肠癌细胞 ,5 3μmol/L亚甲蓝作用 2 4h可杀死全部大肠癌细胞 ,0 2 7μmol/L亚甲蓝即可明显抑制人结肠癌细胞的增殖 .透射电镜观察显示亚甲蓝损伤细胞的部位在线粒体 .研究结果表明 ,亚甲蓝对体外培养的人大肠癌细胞Lovo具有显著的杀伤和抑制作用 ,其损伤癌细胞的部位在线粒体
In order to explore the antitumor effect of methylene blue on human colorectal cancer cells cultured in vitro, the effects of methylene blue on human colorectal cancer cell line Lovo in vitro were studied by direct killing assay, cell growth inhibition assay and colony forming ability assay And the damage site of the drug on the cancer cells was observed by transmission electron microscopy.The results showed that 444% of colorectal cancer cells were killed by 13 2 5 μmol / L methylene blue for 24 hours, while 5 3μmol / L methylene blue The role of 24 h can kill all the colorectal cancer cells, 0 2 7μmol / L methylene blue can significantly inhibit the proliferation of human colon cancer cells.Transmission electron microscopy showed methylene blue damage cells in the mitochondria site.The results show that the methylene Blue in vitro cultured human colorectal cancer cells Lovo has a significant killing and inhibition, the damage of cancer cells in the mitochondria