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此文重点介绍近年来建立起来的另一酶免疫分析技术——酶联免疫斑点 (ELISPOT)技术的基本原理及操作步骤 ,并结合作者实验室多年来的应用经验总结了其优缺点及实验操作中应注意的问题
This article focuses on the establishment of another in recent years, enzyme immunoassay technology - ELISPOT (ELISPOT) technology, the basic principles and operational steps, combined with the author’s laboratory over the years of application experience summarized its advantages and disadvantages and experimental operation Should pay attention to the problem