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目的:本文旨在研究ELISA法丙型肝炎阴性反应板再利用一次的实验,通过实验数据来证实这一使用的可行性。方法:我们课题针对双抗原夹心法测定丙型肝炎表面抗体,阴性包被板再利用一次的研究,规范了阴性再利用包被板的选择方法和使用范围。节约试剂,减少医用垃圾的处理.对临床丙肝阴性患者阴性的包被板,显色后不加酸性终止液,30 min内将其包被板洗净扣干备用。结果:此类包被板当天使用与新试剂无区别不影响检测底线,用其测定HCV血清含量1 ng/ml结果百分之百,但不能长时间保存,保存时间>24 h影响检测底线。结论:可用于复检cutoff周围样本复检及临床常规样本的检测。
Aims: This study aimed to study the ELISA re-use of hepatitis C negative plate once experiment, the experimental data to confirm the feasibility of this use. Methods: Our research aimed at the double antigen sandwich method for the determination of hepatitis C surface antibodies, negative coated plate re-use of a study to regulate the negative re-use of the selection method and the use of coated plate. Save reagents and reduce the disposal of medical waste.A negative coating plate for clinically HCV-negative patients was given no acid stop solution after color development, and the coated plate was washed and set aside for use within 30 min. Results: No differences between this kind of coated plate and the new reagent did not affect the bottom line of detection. The results showed that the serum concentration of 1 ng / ml of HCV was 100%, but could not be stored for a long time. The storage time> 24 h affected the detection bottom line. Conclusion: It can be used to re-examine the samples around the cutoff and clinical routine samples.