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去蛋白灵芝多糖是从芝灵Ganoderma lucidum(Leyss·ex Fr.)Karst子实体中提取的纯多糖部分,根据分子量不同,命名为GL-A,GL-B和GL-C。混合淋巴细胞培养反应是同种异型抗原(主要组织相容性抗原)刺激的特异性免疫反应。GL-A(16～250μg·ml~(-1)),GL～B(62～250μg·ml~(-1)),GL-C(16～250μg·ml~(-1))都可以明显促进混合淋巴细胞培养中脾细胞对[~3H]TdR的摄取。而且促进作用与药物浓度之间呈正相关。三种灵芝多糖还可以在同样条件下增加脾细胞中DNA多聚酶α的活性。但是在无细胞体系中,同样的药物浓度反而抑制[~3H]TTP参入酸不溶物。以上结果提示,去蛋白灵芝多糖可以促进由同种异型抗原刺激的淋巴细胞转化作用。其作用机制是通过间接诱导DNA多聚酶的产生,从而促进免疫细胞中DNA的合成和促进细胞的增殖,加速免疫应答过程。
The deproteinized ganoderma lucidum polysaccharide is a pure polysaccharide fraction extracted from the Karst fruit body of Ganoderma lucidum (Leyss ex Fr.), and is named as GL-A, GL-B, and GL-C depending on the molecular weight. The mixed lymphocyte culture response is a specific immune response stimulated by alloantigens (major histocompatibility antigens). GL-A (16-250 μg·ml -1), GL-B (62-250 μg·ml -1), and GL-C (16-250 μg·ml -1) were all significant Promote uptake of [~3H]TdR by spleen cells in mixed lymphocyte culture. Moreover, there was a positive correlation between the promotion effect and the drug concentration. Three ganoderma lucidum polysaccharides can also increase the activity of DNA polymerase alpha in spleen cells under the same conditions. However, in the cell-free system, the same drug concentration instead inhibits [~3H]TTP incorporation into acid insoluble matter. The above results suggest that deproteinized Ganoderma lucidum polysaccharides can promote lymphocyte transformation stimulated by alloantigens. Its mechanism of action is through the indirect induction of the production of DNA polymerase, which promotes the synthesis of DNA in immune cells and promotes cell proliferation and accelerates the immune response process.