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目的:探讨内质网应激相关因子CHOP/GADD153在多囊卵巢综合征(PCOS)患者卵巢黄体颗粒细胞上的表达。方法:收集因PCOS不孕、行IVF-ET取卵时的黄体颗粒细胞(PCOS组,n=45)及因男方因素不孕、行IVF/ICSI-ET取卵时的黄体颗粒细胞(对照组,n=45),应用RT-PCR和Western blotting方法检测患者卵巢黄体颗粒细胞CHOP/GADD153的表达。结果:PCOS组CHOP/GADD153 mRNA水平(0.45±0.20)及蛋白水平(0.62±0.26)均较对照组(分别为0.40±0.18、0.56±0.17)有增高趋势,但差异均无统计学意义(P>0.05)。结论:内质网应激标志蛋白CHOP/GADD153在PCOS患者卵巢黄体颗粒细胞上的表达无明显改变,推测PCOS患者卵巢黄体颗粒细胞内质网应激尚处于保护性阶段。
Objective: To investigate the expression of endoplasmic reticulum stress-related factor CHOP / GADD153 on corpus luteum granulosa cells in patients with polycystic ovary syndrome (PCOS). Methods: Luteinizing granulosa cells (PCOS group, n = 45) and IVF / ICSI-ET ovariectomized female infertile men (control group , n = 45). The expression of CHOP / GADD153 in ovarian luteal granulosa cells was detected by RT-PCR and Western blotting. Results: The levels of CHOP / GADD153 mRNA (0.45 ± 0.20) and protein (0.62 ± 0.26) in PCOS group were higher than those in control group (0.40 ± 0.18 and 0.56 ± 0.17, respectively), but there was no significant difference > 0.05). Conclusion: Endoplasmic reticulum stress marker protein CHOP / GADD153 in PCOS patients with no significant changes in corpus luteum granulosa cells speculated that PCOS patients ovarian luteinizing granulosa cells endoplasmic reticulum stress is still in a protective stage.