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为探讨脑源性神经营养因子在部分去传入猫脊髓背角的表达状况 ,对成年雄性家猫切除 L1 ～ L5 ,L7～ S2 背根节、保留L6 节及背根 ,术后分别存活 3、6、12 d,对 L6 节段脊髓进行脑源性神经营养因子免疫组织化学反应。结果表明 :正常时 ,阳性反应物主要分布在脊髓 I、II板层的神经终末、膨体与少量神经元胞体内。术后 3 d,术侧 II板层阳性神经终末和膨体密度开始减少 ,6d减少至高峰 ,到第 12 d时两者又均恢复到正常水平 ,而阳性神经元胞体的数量却无明显变化。作者认为 3 d、6d的减少主要与邻近背根节切除后 ,其神经纤维和膨体溃变有关 ;12 d时 ,保留的 L6 背根逐渐侧支出芽 ,且与靶细胞再建功能联系 ,故脑源性神经营养因子阳性神经纤维和膨体数均趋于恢复。说明脑源性神经营养因子既参与脊髓的正常生理功能 ,也与其损伤后的可塑性有关。
To investigate the expression of BDNF in part of the dorsal horn of the spinal cord of cats, L1 ~ L5 and L7 ~ S2 dorsal root segments were excised from adult male domestic cats, and the L6 and dorsal roots were preserved. , 6,12 d, the L6 segment spinal cord brain-derived neurotrophic factor immunohistochemical reaction. The results showed that: Normal, positive reactants are mainly distributed in the spinal cord I, II nerve terminal layer, a small amount of nerve cells and bulk body. At 3 days after operation, the positive terminal of nerve fibers and lumbar densities began to decrease in operation II, and peaked at 6 days. Both of them returned to normal level on the 12th day, while the number of positive neuronal soma was not obvious Variety. The authors concluded that the decrease of 3 d and 6 d was mainly related to the degeneration of nerve fiber and bulge after adjacent dorsal root ganglion resection. At 12 d, the retained L6 dorsal root gradually budded and contacted with target cell rebuilding function. Brain-derived neurotrophic factor-positive nerve fibers and the number of bulge tended to recover. That brain-derived neurotrophic factor both involved in the normal physiological function of the spinal cord, but also with its plasticity after injury.