论文部分内容阅读
目的观察在不同类型造血干细胞移植中巨细胞病毒感染状况。方法应用半巢式PCR技术定期检测了2001-03~2003-07苏州大学附属第一医院住院病人55例移植病人外周血标本,比较不同类型移植CMV-DNA阳性率的差别。结果在检测的462份标本中,有285份为阳性,33/55(60%)患者CMV-DNA阳性,非清髓性造血干细胞移植(NST)的阳性检出率为15/17(88·2%),亲缘间外周血造血干细胞移植(PBSCT)的阳性检出率为3/7(42·9%),非亲缘性骨髓移植(UR-BMT)的阳性检出率为7/11(63·6%),亲缘性骨髓移植(R-BMT)的阳性检出率为8/20(40·0%)。NST与PBSCT、R-BMT的巨细胞病毒感染率差别显著(χ~2=5·44,P<0·05;χ~2=9·14,P<0·05),NST与UR-BMT(χ~2=2·39,P>0·05)间的差别无统计学意义。结论巨细胞病毒感染在不同的造血干细胞移植方式可能存在差异。
Objective To observe the status of cytomegalovirus infection in different types of hematopoietic stem cell transplantation. Methods Semi-nested PCR was used to detect peripheral blood samples from 55 patients with inpatients admitted to the First Affiliated Hospital of Soochow University from 2001-03 to 2003-07. The positive rates of CMV-DNA in different types of transplants were compared. Results Of the 462 specimens tested, 285 were positive and 33 of 55 (60%) were CMV-DNA positive. The positive rate of non-myeloablative stem cell transplantation (NST) was 15/17 (88 · 2%). The positive rate of peripheral blood stem cell transplantation (PBSCT) was 3/7 (42.9%) and the positive rate of non-related bone marrow transplantation (UR-BMT) was 7/11 63.6%). The positive detection rate of R-BMT was 8/20 (40.0%). The difference of cytomegalovirus infection rate between NST and PBSCT and R-BMT was significant (χ ~ 2 = 5.44, P <0.05); χ ~ 2 = 9.14, P < (χ ~ 2 = 2.39, P> 0.05), the difference was not statistically significant. Conclusions CMV infection may be different in different types of hematopoietic stem cell transplantation.