PROTOPLAST相关论文
Rhizoctonia cerealis is the main pathogen of wheat sharp eyespot in our country.In order to obtain high quality protopla......
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Protoplasts of Penicillium novae-zeelandiae HSD07B have been successfully obtained by using lywallzyme on the mycellum.I......
为建立秀珍菇原生质体的高效制备体系,以夏秀作为供试菌株,采用正交试验法研究影响秀珍菇原生质体制备和再生的条件,对菌龄等7个单......
DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (Triticum
Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 1......
Soybean(Glycine max(L.) Merr.), grown for its plant oils and proteins, is one of the most important crops throughout the......
Enhancement of Arachidonic Acid Production by Mortierella isabellina Through Protoplast Regeneration
A developed method was used for the enhancement of arachidonic acid production by M. isabellina. An orthogonal, rotatabl......
对珍稀食药用菌榆耳的原生质体制备与再生的条件进行优化,明确了原生质体制备的最佳条件:采用培养时间为7 d的菌丝,使用0.6 mol/L......
为了探寻橡胶树原生质体培养再生植株的较佳途径,本研究以橡胶树热研7-33-97花药和内珠被为起始外植体分别诱导愈伤组织,进行悬浮......
植物原生质体是进行细胞工程遗传操作和植物育种的的良好材料。由于部分植物的不亲和障碍和特殊细胞信号传导途径研究等方面的需要......
目的 克隆牛乳源致病性金黄葡萄球菌nEBPS基因,经T程菌表达得到其编码抗原纯化蛋白,制备多克隆抗体,采用间接荧光抗体法鉴定nEBPS......
本文通过对荣华二采区10...
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Protoplasts from Candida tropicalis and Candida lipolytica were fused under an optimized electrofusion (electrical pulse......
Cotyledons: a useful biological material for transient gene expression analysis in rapeseed (Brassic
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Gene delivery into isolated Arabidopsis thaliana protoplasts and intact leaves using cationic, a-hel
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Cellular Tolerance, Accumulation and Distribution of Cadmium in Leaves of Hyperaccumulator Picris di
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
Ultrastructure of Single Cells, Callus-like and Monosore-like Cells in Porphyra yezoensis Ueda on Se
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chl
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
A Comparison of Different Gracilariopsis lemaneiformis (Rhodophyta) Parts in Biochemical Characteris
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Dynamic organization of actin cytoskeleton during the polarity formation and germination of pollen p
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
Genetic transformation of marine Actinomycete sp. Isolate M048 and expression of a recombinant plasm
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7......
A preliminary study on the speciation of rare earth elements in plant cells has been carried out by molecular activation......
[目的]探讨米曲霉原生质体制备的高效方法.[方法]统计米曲霉菌丝体检测不同生长时间(72、84、96、108、120 h)的菌丝、不同渗透压(......
[目的]优化冬虫夏草原生质体再生条件.[方法]考察纤维素酶浓度、蜗牛酶浓度、两者混合体积配比、酶解温度、酶解时间、菌龄、稳定......
拟南芥原生质体在超声破碎过滤后,对其叶绿体、细胞核等细胞器进行再生培养.观察到超声破碎对叶绿体有一定破坏作用导致叶绿体在蓝......
Dynamic organization of actin cytoskeleton during the polarity formation and germination of pollen p
The formation of the polarity of pollen protoplast and the dynamics of actin cytoskeleton were observed by non-fixation,......
采用Nd:掺钕的钇铝石榴石固体激光器,在266 nm激发波长下,对绿色木霉CICC13038原生质体进行辐照诱变,通过微晶纤维素筛选,得到高产......
对造成香菇原生质体单核化后两种交配型单核体比例偏离1,1分离的遗传背景进行探讨.结果表明,B因子或是与B因子连锁的因子对这种偏......
Ultrastructure of Single Cells, Callus-like and Monosore-like Cells in Porphyra yezoensis Ueda on Se
It had been demonstrated that individual cells or protoplasts isolated from Porphyra thallus by enzyme could develop int......
考察了细胞壁降解酶的种类、酶解时间、培养基种类和涂布方式对顶头孢霉原生质体制备和再生的影响,结果提示顶头孢霉菌丝形态对原......
The aim of this study is to isolate protoplasts from Undaria pinnatifida. Protoplasts of the alga were isolated enzymati......
Enhancement of Arachidonic Acid Production by Mortierella isabellina Through Protoplast Regeneration
A developed method was used for the enhancement of arachidonic acid production by M. isabellina. An orthogonal, rotatabl......
【目的】为蟹味菇体细胞诱变、融合、基因编辑等研究提供参考。【方法】采取单因素试验和响应面法,对影响蟹味菇原生质体的制备及......
原生质体作为单细胞且具有全能性,为研究植物细胞壁生物合成提供了独特的视角和模型。原生质体细胞壁再生过程复杂,涉及诸多细胞信......
Populus spp.have long been used as model woody plant species for molecular biology research.However,tissues of poplar ar......
Optimization of protoplast isolation,transformation and its application in sugarcane (Saccharum spon
Sugarcane is a prominent source of sugar and ethanol production.Genetic analysis for trait improvement of sugarcane is g......
原生质体融合技术是在没有细胞壁阻碍的条件下,由PEG(聚乙二醇)助融,使两个亲株整套基因相互接触、交换、组合,得到多种类型的重组......
初步研究了原生质体无性系繁殖在光木耳遗传育种中的应用。结果表明与原始菌株相比，光木耳原生质体再生菌株未出现遗传性变异。一些......
对二棱大麦白色胚性无性系细胞与豌豆绿色叶肉细胞原生质体，在ＰＥＧ和Ｃａ的共同作用下，异源融合研究结果表明：将含有２０ｍｍｏｌＣａＣｌ＿２．２Ｈ＿２Ｏ的０．３ｍＬ和０．２ｍｏｌＰＥＧ（２０００）溶液滴入０．１ｍｌ二棱大麦与豌......
采用N-甲基-N′-硝基-N-亚硝基胍(NTG)诱变原生质体,选育了一株产透明质酸的兽疫链球菌(Streptococcus zooepidemicus)H-35.并经过......
以酒曲中筛选到的酵母菌A4为出发菌株,用紫外线对其原生质体进行物理诱变,经过三级筛选得到突变高产菌株A4-20。以麦芽汁为基质发......
从黄瓜无菌苗子叶游离原生质体,培养在含NAA1mgL-1+2,4-D0.5mgL-1+6-BA0.5mgL-1的MS（大量元素减半）和DPD液体培养基中,3d后开始第1次......
采用定向推理筛选方法对龟裂链霉菌原生质体进行诱变筛选得到３４６＃菌株，其复筛时比对照产量高７％，而在６０吨大罐上连续发酵９批，发酵指数比对照高了......
以一株能聚磷但不耐热的芽孢杆菌和一株嗜热但不聚磷的嗜热菌为亲本，PEG诱导原生质体融合，筛选出2株既耐高温又能聚磷的融合菌株。对......
禾谷镰孢是小麦赤霉病和玉米茎腐病的主要病原真菌。试验优化了聚乙二醇介导的原生质体转化法，建立了禾谷镰孢高效转化的稳定体系，并......
利用动物免疫方法制备了啤酒酵母(Saccharomyces cerevisiae)和毕赤氏酵母(Pichia pastoris)的免疫血清.将啤酒酵母和毕赤氏酵母单......
研究了影响L-异亮氨酸（L—Isoleucine）产生菌钝齿棒杆菌（Corynebacterium crenatum）AS1．998原生质体形成和再生的各个因素。结果表明：菌......
以冬虫夏草菌丝体为材料，经原生质体诱变获得了生物量和富硒能力均高于出发菌株的无性型菌株。实验结果表明：经紫外线诱变的8号再生......
针对2株米曲霉进行原生质体制备条件的研究,比较了制备材料、菌龄、渗透压稳定剂、复合酶配比、酶解时间以及酶解温度对米曲霉原生......
