论文部分内容阅读
Members of the Camelidae family produce immunoglobulins devoid of light chains.We have characterized variable domain fragments of these heavy chain antibodies, the VHH, from llamas immunized with human immunodeficiency virus type 1 (HIV-1) envelope protein in order to identify VHH that can inhibit HIV-1 infection.To increase the chances of isolating neutralizing VHH, we employed a functional selection approach, involving panning of phage libraries expressing the VHH repertoire on recombinant gpl20, followed by a competitive elution with soluble CD4.By