Background: microRNA is a small non-coding RNA of~22nt which is essential at posttranscriptional level of gene expression, and is closely related to human disease especially cancer.Yet, the detailed regulatory mechanisms of microRNA in human system is not clear at present.Hear we try to touch upon this problem by constructing the microRNA-TF regulatory networks for different tissues and to find the regulatory features ofmicroRNAs.Methods: We first construct microRNA-TF regulatory network for seven human tissues which include microRNA to target gene regulation and the regulation of transcription factors (TF) to non_TF targets and microRNAs.Then we acquire house-keeping microRNAs and tissue-specific microRNAs, and randomize the network while keeping the degree of each node unchanged.After that, we compare the topological properties such as indegree, outdegree, betweenees, incloseness and outcloseness for house-keeping microRNA and tissue-specific microRNA in the original network and the randomized network to acquire microRNA regulatory features.Results: By comparing to other microRNAs, we find that the tissue-specific microRNAs show higher betweenees, indegree and incloseness.Higher betweenees means that tissuespecific microRNAs tend to bridge many upstream and downstream pathways in gene regulation, and higher indegree means that these microRNAs tend to be regulated by more TFs, while higher incloseness implies that these microRNAs tend to be regulated more quickly.We noticed that the house-keeping microRNAs showed few topological biases in the seven tissues.Conclusions: Compared to other microRNAs, the tissue-specific microRNAs are involved in more pathways in the regulation level to assist the special functions of every tissue, and they would react more promptly when the upstream regulators are interfered.This implies that subtle changes in regulation level could influence tissues certain function through tissue-specific microRNAs.While house-keeping microRNAs mainly maintain common functions for the tissues .