【摘 要】
：
Tumor development has long been known to resemble abnormal embryogenesis.The embryonic stem cell (ESC) self-renewal genes Nanog and Oct4 are purportedly exp
【机 构】
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UniversityofTexasM.DAndersonCancerCenterUSA
【出 处】
：
2008中国深圳蛋白质和多肽科学大会
论文部分内容阅读
Tumor development has long been known to resemble abnormal embryogenesis.The embryonic stem cell (ESC) self-renewal genes Nanog and Oct4 are purportedly expressed by some epithelial cancer cells but their causal roles in tumor development have remained unclear.Here we show that cultured cancer cells,as well as xenograft-and primary tumor-derived cancer cells express both Nanog mRNA and protein.Cancer cell-derived Nanog mRNA,in contrast to expression in ESCs and embryonal carcinoma (EC) cells,is transcribed from a retrotransposed gene and encodes a unique Nanog protein.Nanog-expressing cells are increased in primary prostate tumors and enriched in CD133+ cancer stem cells.RNAi-mediated knockdown of Nanog expression inhibits tumor cell clonal expansion,clonogenic growth and,importantly,tumor development.Ectopic expression of human tumor cell-derived Nanog in transgenic mice leads to abnormal differentiation and hyperplasia.Together,these results demonstrate that a pluripotency gene essential for ESC self-renewal also plays a crucial role in tumorigenesis.
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