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目的采用高效液相色谱法测定色布茹-7中甘草苷的含量。方法甘草苷含量分析采用高效液相色谱法。色谱条件:乙腈-0.5%冰醋酸(18:82)为流动相;检测波长为276 nm,流速1.0 ml/min。结果样品中甘草苷达到基线分离;结果甘草苷在0.09856~0.98560μg范围内呈良好的线性关系(r=1.0000),平均加样回收率为98.5%(RSD=3.1%)。结论所建立的含量测定方法简便、准确、具有实用性。
Objective To determine the content of glycyrrhizin in Sera Ru-7 by high performance liquid chromatography. Methods Glycyrrhizin content analysis using high performance liquid chromatography. Chromatographic conditions: acetonitrile-0.5% glacial acetic acid (18:82) as the mobile phase; detection wavelength of 276 nm, flow rate of 1.0 ml / min. Results The glycyrrhizin was separated from baseline in the sample. Results The calibration curve of glycyrrhizin showed good linearity (r = 1.0000) in the range of 0.09856 ~ 0.98560 μg with an average recovery of 98.5% (RSD = 3.1%). Conclusion The established method for the determination of content is simple, accurate and practical.