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研究姜黄素通过调控miR-29b/KDM2A抑制宫颈癌细胞侵袭和迁移的作用机制.用姜黄素处理HeLa宫颈癌细胞,观察细胞活力、细胞迁移和细胞侵袭情况.我们还评估了microRNA-29b (miR-29b)对HeLa SiHa细胞生物学行为的影响.利用荧光素酶报告基因实验预测和确认miR-29b的潜在靶基因,分析姜黄素和miR-29b对PI3K/AKT信号通路的影响.姜黄素对HeLa细胞的增殖、迁移和侵袭均有抑制作用(P＜0.05).在HeLa细胞中,姜黄素可促进miR-29b的表达(P＜0.01),而miR-29b的下调可恢复姜黄素对HeLa细胞增殖、迁移和侵袭的影响(P＜0.05).KDM2A被证实是miR-29b的直接靶基因,而姜黄素和miR-29b可以调控PI3K/AKT信号通路的活性(P＜0.05).姜黄素对宫颈癌具有有效的保护作用.姜黄素可通过miR-29b/KDM2A/PI3K/AKT通路抑制宫颈癌细胞的增殖、迁移和侵袭.“,”In this study,we aimed to examine whether curcumin exerted its anti-tumor effects by regulating miR-29b/KDM2A in cervical cancer cells.The cell viability,migration and invasion were estimated in HeLa cervical cancer cells treated with curcumin.The effects of microRNA-29b (miR-29b) on biological behaviors of HeLa SiHa cells were also assessed.Potential target genes of miR-29b were predicted and confirmed using a luciferase reporter assay,and the effects of curcumin and miR-29b on the PI3K/AKT signaling pathway were analyzed.Curcumin treatment inhibited cell proliferation,migration and invasion of HeLa cells (P＜0.05).The miR-29b expression was promoted by curcumin treatment in HeLa cells (P＜0.01),and miR-29b depletion could restore the effects of curcumin on cell proliferation,migration and invasion of HeLa cells (P＜0.05).KDM2A was proved as a direct target gene of miR-29b,and the activity of the PI3K/AKT signaling could be regulated by curcumin and miR-29b (P＜0.05).All the data revealed that curcumin played a protective role in cervical cancer.The proliferation,migration and invasion of cervical cancer cells were inhibited by curcumin through the miR-29b/KDM2A/PI3K/AKT pathway.