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近几年来,研究脑内神经递质及神经肽在个体发育中的变化及其作用吸引了许多神经科学家的兴趣,国内这方面的工作也在不断地增加。然而,胚胎脑组织体积小、水份多及递质含量低,给进行免疫细胞化学研究增加了不少困难。我们在近两年研究大白鼠脑内多巴胺神经元发育过程中,获得了用胚胎脑组织进行免疫细胞化学反应的一些经验和体会。现介绍如下:1.在母体子宫内迅速取出胎鼠。在解剖镜下,用眼科镊分离开复盖在心脏表面的组织后,用尖端为50μ～100μ的微玻管插入左心室(稍大胚胎用头皮针)。该微玻管通过尼龙管连接在微量灌流泵上。为
In recent years, the study of neurotransmitters and neuropeptides in the brain development and changes in individual development has attracted many neuroscientists interest in this area of domestic work is also continuously increasing. However, embryonic brain tissue is small in volume, has a lot of water and has a low content of neurotransmitter, which adds a lot of difficulties to immunocytochemistry research. In the past two years, we studied the development of dopamine neurons in the brain of rats and gained some experience and experience in immunocytochemical reaction with embryonic brain tissue. Are introduced as follows: 1. In the mother’s womb quickly remove the fetus. Under the dissecting microscope, the tissue covered with the surface of the heart was separated by using ophthalmic forceps, and the left ventricle was inserted into the left ventricle (slightly scalp needles for scalp) with a microtube with a tip of 50μ to 100μ. The microtube was connected to a micro-perfusion pump through a nylon tube. for