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以连翘根系土壤及不同产地老翘为试材,采用凯氏定氮法、火焰光度计法及2015版药典中醇溶性浸出法及高效液相色谱方法,研究了连翘根系土壤中pH,全氮、速效钾和速效磷含量与药材有效成分含量的相关性。结果表明:连翘酯苷A与土壤因子中全氮、速效磷、速效钾均有显著的相关性,相关系数分别为-0.303*、-0.300*和-0.299*;连翘苷与土壤因子中全氮、速效磷、速效钾则没有显著性;连翘中醇溶性浸出物与速效磷间呈显著负相关,显著系数为-0.312*。通过灰度关联度法分析发现,连翘醇溶性浸出物、连翘酯苷A、连翘苷均与土壤中pH和速效钾关联系数大,连翘酯苷A与速效磷、速效钾、全氮和pH的关联系数为0.852 7、0.851 6、0.844 6和0.844 4,说明连翘酯苷A含量积累与土壤因子间关系密切;连翘苷与速效钾、pH的关联系数分别为0.487 2和0.473 2;醇溶性浸出物与pH和速效钾关联系数为0.721 8和0.514 5,综合上述可以得出连翘有效成分与土壤因子中速效钾、pH关系最为密切,其次为速效磷和全氮。由分析可知连翘根系土壤中N、P、K含量对连翘药材有效成分含量的积累有一定的相关性,在对连翘规范化种植中应合理使用N、P、K的施肥配比。
Forsythia suspensa root soils and different provenances were used as materials to study the effects of Kjeldahl method, flame photometer method and the 2015 version of pharmacopoeia on alcohol-soluble leaching method and high performance liquid chromatography (HPLC) Correlation between content of total nitrogen, available potassium and available phosphorus and active ingredients in medicinal materials. The results showed that forsythoside A was significantly correlated with total nitrogen, available phosphorus and available potassium in soil, the correlation coefficients were -0.303 *, -0.300 * and -0.299 *, respectively. Total nitrogen, available phosphorus and available potassium had no significant difference; forsythia in alcohol-soluble extract and available phosphorus showed a significant negative correlation between the significant coefficient of -0.312 *. Through the gray relational analysis, it was found that forsythia suspense alcohol extract, forsythoside A, forsythin and soil pH and available potassium correlation coefficient, forsythoside A and available phosphorus, available potassium, total The correlation coefficients of nitrogen and pH were 0.852 7,0.851 6,0.844 6 and 0.844 4, indicating that the accumulation of Forsythoside A was closely related to soil factors. The correlation coefficients of forsythin with available potassium and pH were 0.487 2 and 0.473 2. The correlation coefficient of alcohol-soluble extract with pH and available potassium was 0.721 8 and 0.514 5. According to the above, it can be concluded that the effective components of Forsythia suspense had the closest relationship with the available potassium and pH in soil, followed by available phosphorus and total nitrogen. From the analysis we can know that the contents of N, P and K in Forsythia suspensa root soils have a certain correlation with the content of active ingredients in Forsythia suspensa. When applying N, P and K to forsythia, the proportion of fertilizers should be reasonable.