目的对简并寡核苷酸引物PCR(Begenerate oligonucleotide-primed PCR,DOP-PCR)和扩增前引物延伸PCR(Primer extension pre-amplification PCR,PEP-PCR)用于指纹检材DNA检验的价值进行研究。方法建立2种指纹检材DNA模型,分别用普通PCR、DOP技术和PEP技术三种方法对指纹DNA样本进行STR分型,对三种方法的效能进行比较评价。结果实验模型1条件下,三种方法均不能获得满意分型结果;模型2条件下,DOP和PEP两种方法可以增加STR分型的成功率和信息量。结论 DOP和PEP技术必须结合高效的DNA提取技术才能最大程度发挥其检验效能。 OBJECTIVE: To evaluate the value of using degenerate oligonucleotide-primed PCR (DOP-PCR) and pre-amplification primer-extension PCR (PEP-PCR) the study. Methods Two DNA fingerprinting samples were established. STR typing was performed on three fingerprinting samples using ordinary PCR, DOP and PEP respectively. The efficacy of the three methods was compared and evaluated. Results Under the experimental model 1, the three methods could not get the satisfactory classification results. Under the model 2 conditions, the two methods of DOP and PEP can increase the success rate and information amount of STR typing. Conclusion DOP and PEP techniques must be combined with efficient DNA extraction techniques to maximize their testing performance.