论文部分内容阅读
为探讨冠状动脉内放射抑制再狭窄的机制，对２３头小型猪冠状动脉行球囊过度扩张术，其中１２头在术后即刻通过~（１９２）Ｉｒ血管内放射治疗系统局部给予２０Ｇｙ的放射剂量，其余１１头作为对照组。用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）方法定量检测血管组织血小板源生长因子（ＰＤＧＦ－Ｂ）和诱导型一氧化氮合成酶（ｉＮＯＳ）基因表达，并测定血管组织一氧化氮（ＮＯ）的含量。结果：冠状动脉内放射明显抑制了术后３天和术后３０天 ＰＤＧＦ－ＢｍＲＮＡ表达（３８．３％，Ｐ＜０．０１；３９．４％，Ｐ＜０．０５）；明显增加了球囊扩张术后３天及术后３０天血管组织ｉＮＯＳｍＲＮＡ的表达（３２．５％，Ｐ＜０．０１；３４．２％，Ｐ＜０．０５）和ＮＯ的含量（１０２．０％，Ｐ＜０．０１；９０．８％，Ｐ＜０．０１）。
To investigate the mechanism of intracoronary radiation-induced restenosis, balloon-over-dilatation of 23 miniature pig coronary arteries was performed. Twelve of them were given a local dose of 20 Gy via the 192Ir intravascular radiotherapy system immediately postoperatively , The remaining 11 as a control group. The expression of platelet-derived growth factor (PDGF-B) and inducible nitric oxide synthase (iNOS) gene in blood vessels were detected by reverse transcription-polymerase chain reaction (RT-PCR) NO) content. Results: Coronary artery irradiation significantly inhibited PDGF-B mRNA expression (38.3%, P <0.01; 39.4%, P <0.05) at 3 days and 30 days postoperatively. The expression of iNOS mRNA (32.5%, P <0.01; 34.2%, P <0.05) and NO content (102.0%, P <0.01; 90.8%, P <0.01).