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基于静电吸附作用,将荷正电的血红蛋白(Hb)与荷负电的聚丙烯酸修饰的石墨烯(PAA-g-Gr)层层自组装制备了多层复合薄膜({PAA-g-Gr/Hb}n),并将其用于修饰玻碳电极(GCE),从而实现对过氧化氢(H2O2)的检测。利用傅里叶红外光谱(FT-IR)和紫外-可见吸收光谱(UV-Vis)对复合薄膜制备过程进行了考察;利用场发射扫描电镜(FESEM)考察了复合薄膜的表面形貌;通过交流阻抗技术(EIS)探究了膜材料的电化学性质;利用循环伏安法(CV)以及计时电流法(CA)考察了复合薄膜修饰电极对H2O2的电化学响应性能。结果表明,该方法制备的复合薄膜对H2O2的线性检测范围为1×10-6~1×10-4 mol/L(RSD=0.999),检测下限为8×10-7 mol/L。
Based on electrostatic adsorption, multilayers ({PAA-g-Gr / Hb) were prepared by self-assembly of positively charged hemoglobin (Hb) and negatively charged polyacrylic acid modified graphene } n), and used to modify the glassy carbon electrode (GCE), in order to achieve the detection of hydrogen peroxide (H2O2). The preparation process of the composite films was investigated by Fourier transform infrared spectroscopy (FTIR) and ultraviolet-visible absorption spectroscopy (UV-Vis). The surface morphology of the composite films was investigated by field emission scanning electron microscopy (FESEM) Impedance technique (EIS) was used to investigate the electrochemical properties of the membrane material. The electrochemical response of the composite thin film modified electrode to H2O2 was investigated by cyclic voltammetry (CV) and chronoamperometry (CA). The results showed that the linear range of this method for the determination of H2O2 was 1 × 10-6 ~ 1 × 10-4 mol / L (RSD = 0.999) and the detection limit was 8 × 10-7 mol / L.