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目的:观察Ⅱ型糖尿病大鼠经长期喂养后各组织氧化应激水平的改变情况及葡萄籽原花青素提取物(GSPE)长期干预对其的保护作用。方法:将成功建立Ⅱ型糖尿病模型的48只SD大鼠随机分为4组:GSPE低、中、高剂量干预组(分别灌胃125、250、500mg/(kg.d))及糖尿病模型(DM)组;另外12只基础饲料饲养的大鼠作为正常对照组。DM组和正常对照组灌胃等量蒸馏水。连续喂养24周后,取血和脏器,检测各组织的氧化应激指标(超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA))。结果:与正常对照组相比,DM组大鼠血清和肝脏组织中SOD的活性水平显著下降(P<0.05),肝脏和肌肉组织中GSH-Px的活性水平亦显著下降(P<0.05),脑组织中MDA含量明显增高(P<0.05);与DM组相比,GSPE血清、肝脏和脾脏组织中SOD的活性水平显著增加(P<0.05),血清、肝脏、肌肉和脾脏中GSH-Px的活性水平亦显著增加(P<0.05),脾、脑中MDA含量显著下降(P<0.05)。结论:Ⅱ型糖尿病大鼠不同组织的氧化损伤程度不同,GSPE长期干预能够减轻各组织的氧化损伤。
OBJECTIVE: To observe the changes of oxidative stress in tissues of type 2 diabetic rats after long-term feeding and the protective effects of long-term intervention of grape seed procyanidins (GSPE). Methods: Forty-eight Sprague-Dawley (SD) diabetic rats were randomly divided into four groups: low, medium and high dose GSPE intervention groups (125, 250 and 500 mg / (kg.d)) and diabetic model DM) group; another 12 rats fed with basal diet served as normal control group. The DM group and the normal control group were given the same amount of distilled water. Twenty-four weeks after continuous feeding, the blood and organs were taken and the indexes of oxidative stress (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) ). Results: Compared with the normal control group, the activity of SOD in serum and liver decreased significantly (P <0.05) and the activity of GSH-Px in liver and muscle decreased significantly (P <0.05) Compared with DM group, the activity of SOD in serum, liver and spleen of GSPE was significantly increased (P <0.05), and the content of GSH-Px in serum, liver, muscle and spleen (P <0.05). The contents of MDA in spleen and brain decreased significantly (P <0.05). CONCLUSION: The oxidative damage of different tissues in type 2 diabetic rats is different. Long-term intervention with GSPE can reduce the oxidative damage of various tissues.