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【目的】检测SARS并发肺纤维化和骨缺血性坏死患者血清生物标志物。【方法】利用表面加强激光解析电离飞行时间质谱(Surface Enhanced Laser Desorption Ionization-Time Of Flight-Mass Spectrometry,SELDI-TOF-MS)技术研究SARS康复人员的血清蛋白质组学变化,用Ciphergen ProteinChip软件和BioMarker Wizard软件分析蛋白质组数据。【结果】SARS并发肺纤维化和骨缺血性坏死的康复人员血清中有3个特异性分子稳定上调,质荷比(M/Z)分别为5 023、8 606和10 641。与正常人血清蛋白质谱相比,SARS人群3个差异性蛋白质相对含量较高(P=0.02)。【结论】SARS并发肺纤维化和骨缺血性坏死患者可能存在血清生物标志物。
【Objective】 To detect serum biomarkers in patients with pulmonary fibrosis and bone ischemia necrosis. 【Method】 Serum proteomics changes of SARS rehabilitation workers were studied by Surface Enhanced Laser Desorption Ionization-Time Of Flight-Mass Spectrometry (SELDI-TOF-MS). Ciphergen ProteinChip software and BioMarker Wizard software analyzes proteomic data. 【Results】 Three specific molecules in the sera of SARS patients with pulmonary fibrosis and osteois necrosis were stably up-regulated. The mass-to-charge ratios (M / Z) were 5 023, 8 606 and 10 641, respectively. Compared with normal human serum protein profiles, the relative content of three different proteins in SARS was higher (P = 0.02). 【Conclusion】 Serum biomarkers may exist in patients with SARS complicated with pulmonary fibrosis and osteonecrosis.