论文部分内容阅读
目的 :研究与分析乙型肝炎病毒前S1抗原检测意义,并探讨S1抗原与病毒复制间关系。方法 :选取78例乙型肝炎患者进行研究,并采用荧光定量法和酶联免疫吸附法检测患者血清乙型肝炎病毒e抗原(HbeAg)和前S1抗原及乙型肝炎病毒脱氧核糖核酸(HBV-DNA)。结果 :经检测发现,78例患者中,前S1抗原阳性率为78.21%(61/78);HBV-DNA阳性率为87.18%(68/78);HbeAg阳性率为56.41%(44/78),在HbeAg阳性患者中前S1抗原阳性率为79.55%(35/44)、HBV-DNA阳性率为86.36%(38/44),前S1抗原阳性率与HBV-DNA阳性率比较无显著差异。68例HBV-DNA阳性患者中,前S1抗原阳性率为86.76%(59/68)、HbeAg阳性率为57.35%(39/68),前S1抗原阳性率明显高于HbeAg阳性率。结论 :HBV-DNA与乙型肝炎病毒前S1抗原存在紧密联系,同时乙型肝炎病毒前S1抗原是乙型肝炎患者体内复制重要指标。
Objective: To study and analyze the detection of hepatitis B virus pre-S1 antigen and to explore the relationship between S1 antigen and viral replication. Methods: Seventy-eight patients with hepatitis B were enrolled in this study. Fluorescent quantification and enzyme-linked immunosorbent assay were used to detect serum HBeAg and pre-S1 antigen and hepatitis B virus DNA (HBV- DNA). Results: The positive rate of pre-S1 antigen was 78.21% (61/78) in 78 patients; the positive rate of HBV-DNA was 87.18% (68/78); the positive rate of HbeAg was 56.41% (44/78) The positive rate of pre-S1 antigen was 79.55% (35/44) and the positive rate of HBV-DNA was 86.36% (38/44) in HbeAg-positive patients. The positive rate of pre-S1 antigen was not significantly different from that of HBV-DNA positive rate. Among 68 HBV-DNA positive patients, the positive rate of pre-S1 antigen was 86.76% (59/68) and the positive rate of HbeAg was 57.35% (39/68). The positive rate of pre-S1 antigen was significantly higher than that of HbeAg. Conclusion: There is a close relationship between HBV-DNA and pre-S1 antigen of hepatitis B virus and the pre-S1 antigen of hepatitis B is an important index of in vivo replication of hepatitis B virus.