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利用SSR标记技术对40份苜蓿材料(6个雄性不育株系和34个苜蓿品种)的遗传距离进行分析,并利用其中的6个不育株系与14个苜蓿品种测交,进一步对遗传距离(GD)、产量配合力与杂种优势效应进行相关性分析。结果表明,25对SSR引物共扩增出189条谱带,其中多态性条带136条,平均多态性位点百分率为69.23%;40份苜蓿材料的遗传距离为0.1818~0.9091,平均0.4544;各亲本一般配合力及遗传距离均与杂种优势效应存在显著正相关,其中亲本一般配合力与杂种优势的相关性高于遗传距离。因此,仅以SSR遗传距离还不足以准确地组配强优势组合,需结合各性状配合力的分析,以充分发挥杂种优势效应。
SSR markers were used to analyze the genetic distance of 40 alfalfa cultivars (6 male sterile lines and 34 alfalfa cultivars), and 6 male sterile lines and 14 alfalfa cultivars were used for further genetic analysis Distance (GD), yield combining ability and heterosis effect. The results showed that a total of 189 bands were amplified by 25 pairs of SSR primers, of which 136 bands were polymorphic and the average percentage of polymorphic loci was 69.23%. The genetic distances among 40 alfalfa cultivars ranged from 0.1818 to 0.9091 with an average of 0.4544 The general combining ability and genetic distance of each parent had significant positive correlation with the heterosis effect, and the correlation between the general combining ability and heterosis of the parents was higher than the genetic distance. Therefore, SSR genetic distance alone is not enough to accurately match the strong combination, combined with the analysis of the combining ability of the traits, in order to give full play to the heterosis effect.