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【目的】以艾比湖湿地盐节木(Halocnemum strobilaceum)、芦苇(Reed)及盐角草(Salicornia)三种植物为对象,研究其根际土壤氨氧化细菌(AOB)的群落多样性。【方法】利用PCR-RFLP的方法,构建氨氧化细菌amo A基因克隆文库,进行系统发育分析。结合三种植物根际理化因子特点,探讨三种植物根际AOB群落结构组成的特点。【结果】通过序列多态性分析表明,三种植物根际土壤AOB amo A基因克隆文库中的所有序列均属于β亚纲(β-Proteobacteria)中的亚硝化单胞菌属(Nitrosomonas)及Nitrosomonas-like,未发现亚硝化螺菌属(Nitrosospira)。三个AOB amo A基因克隆文库分别包括9个OTUs、12个OTUs和7个OTUs,其文库覆盖度均达99%以上,代表性强。三个文库的丰富度、Chao1指数、ACE指数、Shannon指数均为R>H>S。【结论】三种植物的AOB多样性为芦苇>盐节木>盐角草,并且其最优氨氧化菌群各不相同。本研究为系统认识艾比湖湿地植物根际氨氧化细菌群落多样性和结构组成提供了基础。
【Objective】 The objective of this study was to investigate the community diversity of AOB in the rhizosphere soil of three plant species, Halocnemum strobilaceum, Reed and Salicornia. 【Method】 The clone library of amoxicillin amo A gene was constructed by PCR-RFLP and its phylogenetic analysis was performed. Combined with the characteristics of rhizosphere physico-chemical factors in three plants, the characteristics of the composition of AOB community structure in three plants were discussed. 【Result】 Sequence analysis showed that all the sequences in the cloned library of AOB amo A gene in rhizosphere soil of three plants belonged to Nitrosomonas and Nitrosomonas in β-Proteobacteria, -like, no nitrosospira was found. The three AOB amo A gene clones contained 9 OTUs, 12 OTUs and 7 OTUs, respectively. The library coverage was over 99% with strong representativeness. The abundance of three libraries, Chao1 index, ACE index and Shannon index are all R> H> S. 【Conclusion】 The AOB diversity of three kinds of plants is Phragmites australis> Salix japonicus> Salicornia crassipes, and their optimum ammonia oxidizing bacteria are different. This study provided the basis for systematically understanding the diversity and structure of ammonia-oxidizing bacterial communities in the rhizosphere of Lake Aibi wetlands.