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目的研究Ｐ５３蛋白在血管免疫母细胞性淋巴结病（ＡＩＬ）中的表达及ＤＮＡ含量，探讨ＡＩＬ的发生发展规律、病变性质。②方法应用免疫组化技术检测２０例ＡＩＬ病人的免疫表型及Ｐ５３蛋白表达，并用图像分析技术测定ＤＮＡ含量，同时选择非何杰金淋巴瘤（ＮＨＬ）２０例及淋巴结反应性增生病人１０例作为对比研究。③结果ＡＩＬ中Ｐ５３蛋白阳性表达率为３０％（６／２０），与预后无关。ＤＮＡ含量改变对于病人预后有显著影响（ｔ＝２．８６４，Ｐ＜０．０５）。Ｐ５３蛋白表达、ＤＮＡ含量在ＡＩＬ与ＮＨＬ间差异均有显著性（χ２＝４．９１２，ｔ＝３．５５２，Ｐ＜０．０５）。ＡＩＬ中Ｐ５３蛋白阳性表达者ＤＮＡ含量显著高于阴性表达者（ｔ＝２．２２６，Ｐ＜０．０５）。④结论Ｐ５３蛋白表达及ＤＮＡ含量变化均与ＡＩＬ发生发展及恶性转化有关，ＡＩＬ可能为不同原因、不同性质的疾病共有的临床症候群。
Objective To study the expression and DNA content of P53 protein in angioimmunoblastic lymphadenopathy (AIL), and to investigate the occurrence, development and pathological changes of AIL. 2 Methods Immunohistochemical technique was used to detect the immunophenotype and P53 protein expression in 20 patients with AIL, and DNA content was determined by image analysis technique. At the same time, 20 patients with non-Hodgkin’s lymphoma (NHL) and 10 patients with reactive lymph node hyperplasia were selected. As a comparative study. 3 Results The positive expression rate of P53 protein in AIL was 30% (6/20), which was not related to prognosis. Changes in DNA content had a significant effect on the patient’s prognosis (t=2.864, P<0.05). The difference of P53 protein expression and DNA content between AIL and NHL was significant (χ2=4.912, t=3.552, P<0.05). The DNA content of positive expression of P53 protein in AIL was significantly higher than that of negative expression (t=2.226, P<0.05). 4 Conclusions The changes of P53 protein expression and DNA content are related to the occurrence and development of AIL and malignant transformation. AIL may be a common clinical syndrome shared by different causes and different types of diseases.