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马铃薯不同种、品种及倍性间杂种浆果早期脱落,使杂交籽粒无法成活。将这些授粉后不久脱落的子房采集下来进行离体培养,或将胚取下直接培养。基本培养基为MS,制成液体和固体两种培养基,不加或附加不同种类及水平的激素。胚直接培养是一较好方法,幼胚与子房一同接种只有子房膨大,而幼胚却不再发育。固体培养基效果优于液体培养基。对胚在人工培养前的发育时期进行了解剖研究,证明心形胚是形成完整植株的最早时期。
Potato different species, varieties and ploidy hybrid berries early fall, so that hybrid seeds can not survive. The ovaries, which fall soon after pollination, are harvested for in vitro culture, or the embryos are removed and cultured directly. The basic medium for the MS, made of both liquid and solid medium, without or with different types and levels of hormones. Embryo direct culture is a better way, immature embryos and ovary with only ovary enlargement, and immature embryo is no longer developed. Solid medium is better than liquid medium. Anatomical studies of the embryos at the preimplantation stage showed that the heart-shaped embryos were the earliest stages of the formation of intact plants.