论文部分内容阅读
目的 应用双哌达莫 (DPM)、腺苷 (ADO)与 ADO拮抗剂茶碱 (TH)治疗博莱霉素 (BL E)肺纤维化小鼠 ,观察肺、脾等病理变化 ,探讨肺纤维化发病机理。方法 实验第 1天 10 0只小鼠经气管注入 BL E8.5 mg· kg- 1 ,随机分 BL E、 DPM、 ADO和 TH四组 ,第 2天起分别给予 NS (10 0μl· d- 1 )、 DPM、 ADO和 TH ,剂量均为 5 0 mg· kg- 1 · d- 1 ,共 7天 ,第 4～ 30天内处死动物 ,组织化学法观察肺内纤维变化与脾、胸腺、骨髓病理学改变。结果 BL E组和 TH组第 4～ 6天脾、胸腺髓质与骨髓中许多基质细胞坏死导致组织严重疏松。BL E组第 2 0天后脾与胸腺逐渐萎缩 ,肺与脾内网状纤维大量增加。DPM组与 ADO组第 4～ 6天脾、骨髓坏死基质细胞较少 ,第 30天肺未发生纤维化 ,淋巴器官未萎缩。结论 BL E能致脾、骨髓等基质细胞大量坏死与严重组织疏松。内、外源性 ADO能轻度减少基质细胞坏死 ,促进淋巴组织与造血组织增生 ,抑制 BL E诱导的肺纤维化与淋巴器官萎缩。
Objective To investigate the pathological changes of lung and spleen in mice with bleomycin (BL E) pulmonary fibrosis induced by dipyridamole (DPM), adenosine (ADO) and ADO antagonist theophylline (TH) Pathogenesis. Methods On the first day, 100 mice were randomly divided into 4 groups: BL E, DPM, ADO and TH. The mice were randomly divided into 4 groups: NS (100 μl · d-1 ), DPM, ADO and TH were all given at the dose of 50 mg · kg-1 · d-1 for 7 days. The animals were sacrificed within 4 to 30 days. The changes of lung fiber and spleen, thymus, Neo-Confucianism changes. Results In BL E group and TH group, necrosis of many stromal cells in the spleen, thymus medulla and bone marrow on day 4-6 resulted in severe loosening of the tissue. On the 20th day after BL E group, the spleen and thymus gradually atrophied, and the number of reticular fibers in lung and spleen increased significantly. DPM group and ADO group on the 4th to 6th day spleen, bone marrow necrosis less stromal cells, the first 30 days of lung fibrosis, lymphoid organs did not shrink. Conclusion BL E can cause massive necrosis and severe osteoporosis in spleen and bone marrow stromal cells. Endogenous and exogenous ADO can slightly reduce the necrosis of stromal cells, promote the proliferation of lymphoid tissue and hematopoietic tissue, and inhibit BL E-induced pulmonary fibrosis and atrophy of lymphoid organs.