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目的探讨IL-8在儿童幽门螺杆菌(H.pylori)感染相关性胃炎中的作用。方法82例患儿在胃镜下取胃窦粘膜活检标本,行组织病理学检查,采用酶联免疫吸附试验(ELISA)法检测胃窦粘膜中IL-8的含量。结果H.pylori阳性患儿胃粘膜IL-8含量为(15.2±5.4)pg/mg,H.pylori阴性患儿胃粘膜IL-8含量为(3.3±1.3)pg/mg,两组比较差异有统计学意义(t=13.315,P<0.001)。不同的H.pylori定植密度其IL-8含量差异无统计学意义(t=1.831,P=0.074)。H.pylori阳性患儿活动性炎症及淋巴滤泡检出率和阴性患儿比较差异有统计学意义(χ2值分别为12.286、6.413,P均<0.01)。活动性胃炎胃粘膜IL-8含量为(20.0±5.0)pg/mg,非活动性胃炎胃粘膜IL-8含量为(12.8±3.8)pg/mg;有淋巴滤泡形成胃粘膜IL-8含量为(21.1±3.4)pg/mg,无淋巴滤泡形成胃粘膜IL-8含量为(13.5±4.7)pg/mg;差异有统计学意义(t值分别为5.306、4.942,P均<0.01)。结论儿童感染H.pylori后胃粘膜上皮组织可检测到高浓度的IL-8,IL-8能引起中性粒细胞浸润及淋巴滤泡形成,提示IL-8在H.pylori感染后胃粘膜组织的炎症反应中具有重要作用。
Objective To investigate the role of IL-8 in children with gastritis associated with H. pylori infection. Methods Eighty-two children underwent gastroscopic biopsy of antral mucosa and histopathological examination. The content of IL-8 in gastric mucosa was detected by enzyme-linked immunosorbent assay (ELISA). Results The level of IL-8 in gastric mucosa was (15.2 ± 5.4) pg / mg in H.pylori-positive children and (3.3 ± 1.3) pg / mg in H.pylori-negative children, respectively Statistical significance (t = 13.315, P <0.001). There was no significant difference in the concentration of IL-8 among different H.pylori colonies (t = 1.831, P = 0.074). There were significant differences in the active inflammation and the detection rate of lymphoid follicles among the children with positive H.pylori infection (χ2 = 12.286, 6.413, P <0.01). The content of IL-8 in gastric mucosa of active gastritis was (20.0 ± 5.0) pg / mg, the content of IL-8 in gastric mucosa of inactive gastritis was (12.8 ± 3.8) pg / mg, Was (21.1 ± 3.4) pg / mg, and the content of IL-8 in gastric mucosa without lymphoid follicles was (13.5 ± 4.7) pg / mg with statistical significance (t = 5.306,4.942, P <0.01) . Conclusion High concentrations of IL-8 and IL-8 can be detected in gastric mucosal epithelium after H.pylori infection in children, which can cause neutrophil infiltration and lymphoid follicle formation. It is suggested that IL-8 may play a role in the pathogenesis of H.pylori infection of gastric mucosa Inflammatory reaction has an important role.