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目的 研究miR-128对人皮肤黑素瘤细胞系A375的增殖能力和对PTEN及p-Akt蛋白表达的影响,探讨miR-128影响黑色素细胞增殖的机制.方法 将miR-128 mimics或inhibitor应用阳离子脂质体LipofectamineTM2000转染A375细胞,MTT检测转染后细胞增殖活力变化,Western blot方法检测PTEN及p-Akt蛋白表达.结果 转染miR-128 mimics后,A375细胞增殖活力明显增强,p-Akt蛋白表达明显上调,PTEN蛋白表达显著降低;转染miR-128 inhibitor后,A375细胞增殖活力明显降低,p-Akt蛋白表达明显下调,PTEN蛋白表达显著升高.结论 miR-128促进人黑素瘤细胞A375增殖可能与调控PTEN及p-Akt的表达相关.“,”Objective To investigate the effects of miR-128 on the proliferation of human melanoma cell line A375 and expression of PTEN and p-Akt in cell line A375.Methods miR-128 mimics or inhibitor were transfected into A375 cells by lipofectamine package,the cell viability was analyzed by MTT assay,and Western blot was employed to detect the expressions of PTEN and p-Akt protein.Results miR-128 mimics significantly promoted A375 cells proliferation,markedly upregulated p-Akt expression level,but downregulated PTEN expression level in cell line A375.miR-128 inhibitor significantly inhibited A375 cells proliferation,downregulated p-Akt expression level,but upregulated PTEN level markedly cell line A375.Conclusion The promotion of A375 cells proliferation by MiR-128 might be related to the regulation of PTEN and p-Akt expressions.