用机械、酶消化加不连续密度梯度离心的方法从C57BL/6小鼠的实体瘤(B16黑色素瘤和Lewis肺癌)中分离到TIL,发现刚分离的TIL产生IL-2、IFN-γ和淋巴毒素(LT)的能力及其自然杀伤活性均显著低下,已初步证明刚分离的TIL的功能低下状态与TIL在肿瘤微环境中受到肿瘤细胞分泌的免疫抑制因子的作用有关。加IL-2(500U/ml)培养后可以解除TIL的功能低下状 TIL was isolated from solid tumors (B16 melanoma and Lewis lung carcinoma) of C57BL/6 mice by mechanical, enzymatic digestion and discontinuous density gradient centrifugation. It was found that newly isolated TIL produced IL-2, IFN-γ, and lymph The ability of the toxin (LT) and its natural killing activity are both significantly lower, and it has been initially demonstrated that the dysfunctional state of the newly isolated TIL is related to the role of TIL in the tumor microenvironment by the immunosuppressive factors secreted by the tumor cells. Addition of IL-2 (500U/ml) can relieve the hypofunction of TIL after culture