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目的探讨肝细胞生长因子(HGF)下游分子ezrin对肝癌细胞生长和转移的影响,为肝癌转移治疗提供新的思路和理论基础。方法选取同一亲本来源的低转移细胞系SMMC7721和高转移细胞系SF7721为研究对象,首先运用免疫荧光、逆转录-聚合酶链反应(RT-PCR)和Western blot比较ezrin和actin的表达差异。进一步应用RNA干扰下调SF7721中ezrin表达,观察其运动和侵袭能力的改变。结果SF7721的ezrin和F-actin表达明显高于SMMC7721,增高的actin为γ-actin。下调ezrin后,SF7721的生长(分裂期：28．07％下降到23．53％)和侵袭能力[穿膜细胞数：(47,75±4．46)个／视野下降到(31．75±2．81)个／视野]均显著下降(P＜0．05),与SMMC7721的生长和转移差异无统计学意义(P＞0．05)。结论ezrin与HGF介导的肝癌生长侵袭相关,有可能成为抑制肝癌复发转移的新靶点。
Objective To investigate the effect of ezrin, a downstream molecule of hepatocyte growth factor (HGF), on the growth and metastasis of hepatocellular carcinoma (HCC) cells and to provide new ideas and theoretical basis for the metastatic treatment of HCC. Methods SMMC7721 and SF7721 cell lines from the same parental origin were selected for the study. The expression of ezrin and actin were compared by immunofluorescence, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. RNAi was used to further down-regulate the expression of ezrin in SF7721 and to observe its changes in motility and invasion ability. Results The expression of ezrin and F-actin in SF7721 was significantly higher than that in SMMC7721 and the increased actin was γ-actin. After ezrin down-regulation, the growth of SF7721 (split stage: 28.07% down to 23.53%) and invasive ability [cell number per cell: (47,75 ± 4.46) / field down to (31.75 ± (P <0.05). There was no significant difference between SMMC7721 and the growth and metastasis (P> 0.05). Conclusion ezrin is related to HGF-mediated growth and invasion of hepatocellular carcinoma and may be a new target of inhibiting the recurrence and metastasis of hepatocellular carcinoma.