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目的探讨热休克蛋白70(HSP70)-Hom基因多态性与广西巴马人群长寿家族的相关性。方法于2010年7月,选取广西巴马县长寿人群后代101例(22~83岁)为有长寿家族史组,南丹县无长寿家族史的健康人群176例(20~82岁)为无长寿家族史组。对研究对象进行问卷调查和体格检查,采用TaqMan探针实时荧光定量PCR技术对HSP70-Hom基因rs2227956位点进行基因分型。结果有长寿家族史组和无长寿家族史组人群HSP70-Hom基因rs2227956位点基因型和等位基因频率分布差异具有统计学意义(P<0.05)。经多因素logistic回归分析后,TT、CT基因型携带者(ORTT=5.161,95%CI:1.300~20.489;ORCT=2.245,95%CI:1.032~4.883)和高密度脂蛋白胆固醇升高者(OR=6.924,95%CI:2.484~19.301)有长寿家族史的比例较高,体质指数偏高者有长寿家族史的比例较低(OR=0.271,95%CI:0.092~0.796)。结论本研究初步认为有家族性长寿史者可能携带HSP70-Hom基因rs2227956位点的TT、CT基因型,且高密度脂蛋白胆固醇较高,体质指数偏低。
Objective To investigate the association between heat shock protein 70 (HSP70) -Hom gene polymorphism and longevity in Guangxi Bama population. Methods In July 2010, 101 (22-83 years) offspring of longevity population in Bama County of Guangxi were selected as the healthy family with longevity family history and Nandan county without life history. 176 (20-82 years) Longevity family history group. The subjects were surveyed and physical examination, and TaqMan probe real-time fluorescence quantitative PCR was used to genotype the rs2227956 site of HSP70-Hom gene. Results There was statistically significant difference in genotype and allele frequencies of rs2227956 in HSP70-Hom gene between long-life family history group and non-long-life family history group (P <0.05). After multivariate logistic regression analysis, TT and CT genotype carriers (ORTT = 5.161, 95% CI: 1.300-20.489; ORCT = 2.245, 95% CI: 1.032-4.883) and high density lipoprotein cholesterol OR = 6.924, 95% CI: 2.484 ~ 19.301) had a higher proportion of family history of longevity and higher proportion of body mass index had lower family history of longevity (OR = 0.271, 95% CI: 0.092-0.796). Conclusions This study preliminarily concluded that patients with familial longevity may carry TT and CT genotypes of rs2227956 locus of HSP70-Hom gene, with high HDL cholesterol and low body mass index.