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目的探讨子宫内膜HOXA10、白血病抑制因子(LIF)mRNA表达水平与体外受精-胚胎移植(IVF/ICSI-ET)妊娠结局的关系。方法前瞻性收集了行IVF/ICSI-ET者59例,其中21例临床妊娠者作为妊娠组,38例未妊娠者作为非妊娠组,于前1个月经周期的黄体中期取内膜,应用RT-PCR技术检测子宫内膜HOXA10 mRNA和LIF mRNA的表达水平。结果妊娠组与未妊娠组黄体中期子宫内膜LIF mRNA水平分别为1.12±0.32和0.94±0.14,差异有统计学意义(P<0.05);妊娠组与未妊娠组分泌中期子宫内膜HOXA10 mRNA水平分别为0.68±0.12和0.62±0.07,差异有统计学意义(P<0.05)。结论分泌中期子宫内膜HOXA10 mRNA和LIF mRNA表达水平与体外受精-胚胎移植妊娠结局有相关性。
Objective To investigate the relationship between endometrial HOXA10, leukemia inhibitory factor (LIF) mRNA expression and pregnancy outcome of IVF / ICSI-ET. Methods Fifty-nine patients with IVF / ICSI-ET were prospectively enrolled. Among them, 21 cases of clinical pregnancy were as pregnancy group and 38 cases of non-pregnancy as non-pregnancy group. The endometrium was taken from the mid luteal phase of the first menstrual cycle. RT The expression of HOXA10 mRNA and LIF mRNA in endometrium was detected by PCR. Results The levels of LIF mRNA in the endometrium of pregnant women and non-pregnant women were 1.12 ± 0.32 and 0.94 ± 0.14, respectively (P <0.05). The levels of HOXA10 mRNA in endometrium of pregnancy group and non-pregnancy group Respectively, 0.68 ± 0.12 and 0.62 ± 0.07, the difference was statistically significant (P <0.05). Conclusion The expression level of HOXA10 mRNA and LIF mRNA in the endometrial secretion is correlated with the pregnancy outcome of in vitro fertilization and embryo transfer.