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目的: 观察低强度脉冲电磁场 (pulsedelectromag neticfields,PEMFs)对体外培养成骨细胞增殖、分化、体外骨形成的影响.方法: 取出生后( <24h)的SD仔鼠 2只,骨组织块培养法分离、培养成骨细胞.频率 15Hz,场强 8Gs的PEMFs作用于成骨细胞.应用MTT方法、碱性磷酸酶测定、四环素荧光标记检测成骨细胞增殖、分化、体外骨形成情况.结果:①MTT检测结果显示对照组A490nm值为(0 330±0 020),实验组A490nm值为 ( 0 379±0 038 ),两组间有显著差异 (P<0 01).②ALP检测结果显示对照组A410nm值为 ( 0 170±0 029),实验组A410nm值为 (0 206±0 018),两组间有显著差异(P<0 01 ).③钙结节数量实验组也明显多于对照组.结论: PEMFs作用促进成骨细胞增殖、分化、加速了体外骨形成.
OBJECTIVE: To observe the effect of pulsedelectromag neticfields (PEMFs) on the proliferation, differentiation and in vitro bone formation of cultured osteoblasts in vitro.Methods: Two SD pups born after birth (<24h) The osteoblasts were isolated and cultured.PEMFs with a frequency of 15 Hz and field intensity of 8Gs were used to act on osteoblasts.MTT method, alkaline phosphatase assay and tetracycline fluorescent labeling were used to detect osteoblast proliferation, differentiation and in vitro bone formation.Results: ① MTT The results showed that the A490nm value of the control group was (0 330 ± 0 020) and the A490nm value of the experimental group was (0 379 ± 0 038), with significant difference between the two groups (P <0.01) .②The results of ALP showed that A410nm (0 170 ± 0 029), A410nm value of the experimental group was (0 206 ± 0 018), there was a significant difference between the two groups (P <0.01) .③The number of calcium nodules in the experimental group was also significantly more than the control group. : PEMFs promote the proliferation and differentiation of osteoblasts and accelerate the in vitro bone formation.