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目的观察痹肿消汤对大鼠胶原诱导性关节炎肿瘤坏死因子-(αTNF-α)mRNA的影响,从基因水平探讨类风湿关节炎的发病机制及痹肿消汤对胶原诱导性关节炎大鼠的抗炎作用。方法将34只大鼠随机分为3组,皮下注射II型胶原蛋白配合完全弗氏佐剂诱导关节炎模型,采用半定量RT-PCR法测定胶原诱导性关节炎大鼠滑膜细胞TNF-αmRNA的表达。结果TNF-αmRNA在正常组有少量表达;25、45 d模型组TNF-αmRNA表达明显高于正常组(P<0.05),45 d模型组表达高于25 d模型组(P<0.05);给药后,痹肿消汤组的TNF-αmRNA表达明显低于模型组(P<0.05),在痹肿消汤组中,前后比较,45 d TNF-αmRNA的表达低于25 d(P<0.05)。结论在胶原诱导性关节炎大鼠中痹肿消汤能减少TNF-αmRNA的表达,提示痹肿消汤在治疗类风湿性关节炎中有抗炎作用,从而达到治疗作用。
Objective To observe the effect of Qizhongxiao decoction on collagen-induced arthritis in rats with tumor necrosis factor-α (TNF-α) mRNA. To explore the pathogenesis of rheumatoid arthritis from the level of gene and to discuss the effect of Qizhongxiao decoction on collagen-induced arthritis. The anti-inflammatory effects of mice. Methods Thirty-four rats were randomly divided into 3 groups and subcutaneously injected with type II collagen and complete Freund’s adjuvant-induced arthritis models. Semiquantitative RT-PCR was used to determine the TNF-α mRNA in synoviocytes of collagen-induced arthritis rats. expression. Results The expression of TNF-α mRNA was slightly expressed in the normal group. The expression of TNF-α mRNA in the model group was significantly higher than that in the normal group at 25 and 45 d (P<0.05). The expression of TNF-α mRNA in the model group was higher than that in the 25 d model group at 45 d (P<0.05). After treatment, the expression of TNF-α mRNA in the Biaozhongxiao Decoction group was significantly lower than that in the model group (P<0.05). In the Cangzhongxiao Decoction group, the expression of TNF-α mRNA was lower than 25 d after 45 days (P<0.05). ). Conclusion Bianzhongxiao Decoction can reduce the expression of TNF-αmRNA in rats with collagen-induced arthritis, suggesting that Biaozhongxiao Decoction has anti-inflammatory effects in the treatment of rheumatoid arthritis, so as to achieve therapeutic effects.