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分别以大白菜TuMV国家级抗源材料8407和高感TuMV的核心种质材料冠291为亲本构建分离群体,为验证TuMV抗性基因TuRBCS01两侧紧密连锁的分子标记m Br4055和Br ID10723的检测准确率,对上述两个亲本F_2代分离群体的107个单株进行自交构建F_(2∶3)家系,并对每个家系的TuMV抗性进行鉴定,以判断原F_2单株的TuMV抗性及基因型,同时利用上述两个标记引物对F_2代107个单株进行检测,根据检测结果及抗病性鉴定结果计算标记检测的准确率。结果表明,两标记均检测为纯合抗病的株系有22株,其中有2株经抗病性验证为杂合抗病,其余均为纯合抗病,检测准确率为90.9%;两标记均检测为杂合抗病的有48株,经抗病性验证,其中5株为纯合抗病,5株为纯合感病,其余均为杂合抗病,检测准确率为79.2%;两标记均检测为纯合感病的有23株,经抗病性验证,其中4株为杂合抗病,1株为纯合抗病,鉴定准确率为78.3%。上述检测结果为更好地利用标记mBr4055和BrID10723进行分子标记辅助选择奠定了基础。
In order to confirm that the closely linked molecular markers m Br4055 and Br ID10723 on both sides of TuRV resistance gene TuRBCS01 were successfully constructed with Chinese cabbage TuMV national-level resistant material 8407 and high-sensitivity TuMV core 291 as parents, The F 2 (3) lines were constructed from 107 individuals of the F2 progeny of the two parents, and their TuMV resistances were identified to determine the TuMV resistance of the original F 2 plants At the same time, 107 F_2 plants were detected by using the above two primers, and the accuracy of the marker detection was calculated according to the test results and the identification results of disease resistance. The results showed that there were 22 strains tested homozygous for both markers, of which 2 were tested for disease resistance by heterozygosis and the others were homozygous and disease resistant with the detection accuracy of 90.9%. Two 48 were detected as heterozygous and disease-resistant, and 5 were homozygous and resistant, 5 were homozygous and the rest were all heterozygous, the detection accuracy was 79.2% ; 23 markers were detected as homozygous susceptible, tested by disease resistance, of which 4 were heterozygous disease resistance, a homozygous disease resistance, the identification accuracy was 78.3%. The above results provide the basis for better use of the markers mBr4055 and BrID10723 for molecular marker-assisted selection.