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目的研究变异型IκBα(IκBαM)基因在人类多形性胶质母细胞瘤(GBM)中对血管新生的抑制作用。方法选用IκBαM基因转染的胶质瘤细胞株,通过制备血管新生及异位肿瘤生成两组动物模型,检测新生血管的数量及肿瘤中血管生长因子VEGF及IL-8的表达,分析IκBαM对肿瘤中血管新生的作用。结果IκBαM基因转染的人类GBM细胞中,新生血管数量显著减少,诱导形成的异位胶质瘤中的VEGF及IL-8的表达量也显著降低。结论IκBαM基因可有效抑制胶质瘤中的血管新生从而抑制肿瘤的生长,这可作为人类多形性胶质母细胞瘤抗血管治疗的理论基础。
Objective To investigate the inhibitory effect of mutant IκBα (IκBαM) gene on angiogenesis in human glioblastoma multiforme (GBM). Methods Glioma cell lines transfected with IκBαM gene were used to detect the number of neovascularization and the expression of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) in angiogenesis and ectopic tumors. The effects of IκBαM on tumor The role of angiogenesis. Results The number of neovascularization was significantly decreased in human GBM cells transfected with IκBαM gene, and the expression of VEGF and IL-8 in ectopic glioma was also significantly decreased. Conclusion The IκBαM gene can effectively inhibit angiogenesis in gliomas and thus inhibit tumor growth. This may serve as a theoretical basis for the anti-angiogenic therapy of human glioblastoma multiforme.