论文部分内容阅读
目的:研究人乳头状瘤病毒(HPV)感染与鼻内翻性乳头状瘤(IP)发生的相关性,进一步明确HPV57在IP发生中的作用。方法:随机收集经病理确诊的IP病变组织石蜡包埋标本60个,分别采用HPVL1通用引物MY09/11和HPV57型特异性引物进行多聚酶链反应(PCR),对标本的HPV相关DNA序列扩增,计算其阳性率;另取10个慢性鼻窦炎病变组织标本作为对照,采用HPVL1通用引物MY09/11及HPV57型特异性引物进行PCR扩增。结果:60个鼻内翻性乳头状瘤组织标本经HPVL1通用引物MY09/11扩增后,均为阴性。经HPV57型特异性引物扩增后有47个(78.3%)为阳性。10个慢性鼻窦炎标本DNA模板均未能扩增出DNA片断。结论:HPV57型感染可能在IP的发生中起重要作用。
Objective: To study the correlation between human papillomavirus (HPV) infection and nasal inverted papilloma (IP) and further clarify the role of HPV57 in the pathogenesis of IP. Methods: Sixty paraffin-embedded specimens of pathologically confirmed IP lesions were randomly collected. Polymerase chain reaction (PCR) was performed using HPV L1 general primers MY09 / 11 and HPV57 specific primers respectively. The HPV-related DNA sequences were amplified, The positive rate was calculated. Ten other specimens of chronic sinusitis were used as controls, and PCR amplification was carried out by using specific primers HPV L1 universal primer MY09 / 11 and HPV57. Results: Sixty-four cases of nasal inverted papilloma were all negative when amplified by HPV L1 universal primer MY09 / 11. 47 (78.3%) were positive after amplification with HPV57-specific primers. Ten chronic sinusitis specimens DNA template failed to amplify the DNA fragment. Conclusion: HPV57 infection may play an important role in the pathogenesis of IP.