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目的 :探讨内皮细胞功能紊乱在肝缺血再灌注损伤中的作用及川芎嗪对其的影响。方法 :实验兔和肝癌手术患者各分为肝缺血再灌注组和肝缺血再灌注加川芎嗪治疗组 ,分别测缺血前、缺血 45分钟 (或2 5分钟 )和再灌注 45分钟 (或 2 5分钟 )共 3个时相点的血浆透明质酸 ( HA)和丙氨酸转氨酶 ( AL T)活性。结果 :肝缺血再灌注期间 ,HA、AL T明显升高 ,尤以再灌注 45分钟 (或 2 5分钟 )为著〔缺血 45分钟或 2 5分钟 ,家兔 HA为 ( 1.12± 0 .16 )μg/ L ,P <0 .0 1,AL T为 ( 788.49± 76 .6 8) nmol· s- 1 · L- 1 ,P <0 .0 1;患者 HA为( 0 .5 1± 0 .13)μg/ L ,P<0 .0 1,AL T为 ( 15 85 .32± 2 0 3.37) nm ol· s- 1 · L- 1 ,P<0 .0 1。再灌注 45分钟或 2 5分钟 ,家兔 HA为 ( 2 .0 3± 0 .6 5 )μg/ L ,P均 <0 .0 1,AL T为 ( 10 2 8.5 4± 138.36 ) nmol· s- 1 · L- 1 ,P均 <0 .0 1;患者 HA为 ( 0 .5 9± 0 .14) μg/ L,P<0 .0 5和 P<0 .0 1,AL T为 ( 2 10 5 .42± 2 5 1.72 ) nm ol· s- 1· L- 1 ,P均 <0 .0 1〕,川芎嗪可逆转上述指标〔缺血 45分钟或 2 5分钟 ,家兔 HA为 ( 1.0 5± 0 .2 1) μg/ L ,P>0 .0 5 ,AL T为( 6 75 .14± 10 5 .0 2 ) nmol· s- 1· L- 1 ,P>0 .0 5 ;患者 HA为 ( 0 .40± 0 .0
Objective: To investigate the role of endothelial dysfunction in hepatic ischemia-reperfusion injury and the effect of ligustrazine on it. Methods: Experimental rabbits and patients with hepatocellular carcinoma were divided into hepatic ischemia-reperfusion group and hepatic ischemia-reperfusion plus ligustrazine treatment group. Preischemia, ischemia 45 minutes (or 25 minutes) and reperfusion 45 minutes (Or 25 minutes) a total of three time points of plasma hyaluronic acid (HA) and alanine aminotransferase (AL T) activity. Results: During the period of ischemia-reperfusion, the HA and AL T were significantly elevated, especially at 45 minutes or 25 minutes after reperfusion ([HA] was (1.12 ± 0.50) at 45 minutes or 25 minutes after ischemia. 16) μg / L, P <0.01, ALT was (788.49 ± 76.68) nmol · s-1 · L-1, P <0.01; 0.13) μg / L, P <0.01, and AL T was (15 85.32 ± 2.03.37) nm ol · s-1 · L-1, P <0.01. The rabbit HA was (2.03 ± 0.65) μg / L for 45 minutes or 25 minutes after reperfusion, both P <0.01 and ALT was (102.54 ± 138.36) nmol · s - 1 · L-1, all P <0.01; patients with HA were (0.59 ± 0.14) μg / L, P <0.05 and P <0.01, ALT was ( 2 10 5 .42 ± 2 5 1.72) nm ol · s-1 · L-1, P all <0.01), ligustrazine can reverse the above indicators [ischemia 45 minutes or 25 minutes, rabbits HA (1.05 ± 0.21) μg / L, P> 0.05, ALT was (6 75.14 ± 10.55) nmol · s-1 · L-1, P> 5; patient HA was (0.40 ± 0 .0