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目的 应用抗肿瘤坏死因子 (TNF)单克隆抗体 (McAb)免疫吸附方法清除循环TNF ,观察其对内毒素休克兔肝脏改变的影响。方法以致死剂量内毒素 (8× 10 9CFU/kg)制成内毒素休克兔模型后分为对照组 (n =7)及免疫吸附组 (n =10 )。注射内毒素 1h后开始体外循环免疫吸附 ,监测平均动脉压 (MAP) ,观察两组循环血TNF及丙氨酸转氨酶 (ALT)、天门冬氨酸转氨酶 (AST)水平。于 6h后活杀动物 ,留取肝脏行组织病理检查。结果 (1)免疫吸附组 90min后MAP开始回升 [(11 6±1 4)kPa],此后一直明显高于 [(9 2± 2 0 )kPa]对照组 (P <0 0 5 ) ;(2 )免疫吸附组 2h的TNF活性 [(43 6± 10 4)× 10 3 U/L]较对照组 [(144 8 6± 2 2 6 9)× 10 3 U/L]明显降低 (P <0 0 5 )。 (3)免疫吸附组血清ALT、AST水平较对照组降低 (P <0 0 5 )。 (4)免疫吸附组肝脏病理损害程度较对照组明显减轻。结论抗TNFMcAb特异性免疫吸附方法 ,能有效清除循环TNF ,减轻肝脏的病理损害程度。
Objective To investigate the effect of anti-tumor necrosis factor (TNF) monoclonal antibody (McAb) immunosorbent assay on the removal of circulating TNF in liver of endotoxic shock rabbits. Methods Rabbits with endotoxic shock were treated with lethal dose of endotoxin (8 × 10 9 CFU / kg) and divided into control group (n = 7) and immunoadsorption group (n = 10). After 1h injection of endotoxin, cardiopulmonary bypass (IAP) was started and the mean arterial pressure (MAP) was monitored. The levels of TNF, ALT and AST in the two groups were observed. Animals were sacrificed after 6h, and the liver was removed for histopathological examination. RESULTS: (1) MAP began to rise after 90min in the immunoadsorption group [(11 6 ± 1 4) kPa], and was significantly higher than [(9 2 ± 20) kPa] (P <0 05) ) In the immunostaining group was significantly lower than that in the control group [(144 8 6 ± 2 269) × 10 3 U / L] (43 6 ± 10 4 × 10 3 U / L] 0 5). (3) Serum levels of ALT and AST in the immunoadsorption group were lower than those in the control group (P <0.05). (4) The degree of liver pathological damage in immunoadsorption group was significantly lower than that in control group. Conclusion Anti-TNFMcAb specific immunosorbent assay can effectively remove circulating TNF and reduce the degree of liver pathological damage.