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目的 :探讨细菌脂多糖 (LPS)对正常成人外周血单核细胞来源的树突状细胞 (Dendritic cell,DC)表面分子表达及免疫功能的影响。方法 :分离正常人 (n=5 )外周血单个核细胞 (PBMC)以 GM-CSF、IL-4联合诱生 DC,实验组在培养的第 2天加入LPS,正常对照组不加。于第 4、8天 FACS检测 DC表面分子 CD1a、CD14、CD83、CD80、HL A-DR的表达。同期检测 DC的增殖和刺激同种异体 T淋巴细胞的增殖情况。结果 :经 LPS刺激后 ,正常人 DC早期、晚期表面分子 CD1a、CD83、CD80表达增高 ,CD14表达降低 (P<0 .0 5 )。DC数量的增加及刺激同种异体 T细胞能力明显增强。结论 :GM-SF、IL-4和 L PS联合刺激能促进正常人 DC的成熟和免疫功能的提高
Objective: To investigate the effect of bacterial lipopolysaccharide (LPS) on the expression of the surface molecules and the immune function of dendritic cells (DCs) derived from normal adult peripheral blood mononuclear cells. Methods: Peripheral blood mononuclear cells (PBMCs) from normal subjects (n = 5) were induced by combining GM-CSF and IL-4. LPS was added to the experimental group on the second day of culture, while the control group did not. The expression of CD1a, CD14, CD83, CD80 and HL A-DR of DCs were detected by FACS on the 4th and 8th days. In the same period, the proliferation of DCs and the proliferation of allogeneic T lymphocytes were detected. Results: After stimulated by LPS, the expression of CD1a, CD83 and CD80 on early and advanced DCs increased and the expression of CD14 decreased (P <0.05). DC increased the number and stimulate allogeneic T cells significantly enhanced. Conclusion: The combined stimulation of GM-SF, IL-4 and L PS can promote the normal DC’s maturation and the enhancement of immune function