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为从吸收的角度考察干姜对乌头类双酯型生物碱的解毒机理,采用外翻肠囊法展开实验.利用超高液相与三重四极杆质谱联用技术定量检测双酯型生物碱成分,采用标准曲线法计算乌头碱、中乌头碱、次乌头碱在肠囊内吸收的绝对含量,采用质谱峰面积直接分析其它双酯型生物碱的相对变化,结果加入干姜提取液后,乌头碱、中乌头碱、次乌头碱的单位肠管面积累计吸收量均降低,10-羟基中乌头碱的的累积峰面积降低;加入维拉帕米后,双酯型生物碱的单位肠管面积累计吸收量及累积峰面积均增加;向含有地高辛的肠营养液中加入干姜提取液后,地高辛在各实验时间点的单位肠管面积累计吸收量均降低,根据以上结果推测干姜抑制乌头类双酯型生物碱在大鼠肠囊内吸收的可能机制是通过诱导肠内P-葡糖蛋白,从而抑制作为P-葡糖蛋白底物的双酯型生物碱的吸收,最终起到减毒作用.
In order to investigate the detoxification mechanism of Aconitum diterpenoid alkaloids from the perspective of absorption, the experiment was carried out by valgus gut method.Double ester type bioassay was performed by using ultra-high-liquid and triple quadrupole mass spectrometry Alkali composition, using standard curve method to calculate the aconitine, mesaconitine, hypaconitine absorption in the intestine of the absolute content, mass spectrometry peak area direct analysis of other diester alkaloids relative changes, the results of adding ginger After the extract, aconitine, mesaconitine, hypaconitine per unit area of intestine intestinal absorption decreased, the peak area of 10-hydroxy mesaconitine decreased; added verapamil, the diester Alkaloids per unit area of intestine accumulated uptake and cumulative peak area increased; to digoxin-containing enteral nutrition solution after adding ginger extract, digoxin cumulative absorption per unit area of intestine at each experimental point were According to the above results, it is speculated that the possible mechanism of dry ginger inhibiting the absorption of aconitine type diacylglycosides in the intestine of rats is through the induction of p-glucoprotein in the intestine and thus the inhibition of bis Ester-type alkaloid absorption, eventually starting Attenuation.