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目的:探讨XCL1、γ干扰素的浓度变化与乙型肝炎慢性化的关系。方法:采用酶联免疫吸附法(ELISA)检测血清XCL1、IFN-γ的浓度;流式细胞仪检测T淋巴细胞亚群;时间分辨荧光分析仪检测乙型肝炎抗原/抗体半定量指标;全自动生化仪检测肝功能。结果:(1)随着肝脏炎症程度的加重,血清中XCL1的含量逐渐增加。HBeAg+组与HBeAg-组比较,HBeAg+组血清XCL1浓度显著高于HBeAg-组(P<0.05);HBeAg-组血清IFN-γ浓度显著高于HBeAg+组(P<0.05)。(2)血清XCL1浓度与CD4+T细胞含量呈负相关(r=-0.336,P=0.017),IFN-γ浓度与CD8+T细胞含量呈正相关(r=0.319,P=0.024)。结论:γ干扰素通过影响XCL1的表达促进乙型肝炎慢性化的形成与发展,XCL1参与了乙型肝炎慢性化进程。
Objective: To investigate the relationship between the changes of XCL1 and IFN-γ and the chronicity of hepatitis B. Methods: Serum levels of XCL1 and IFN-γ were detected by enzyme-linked immunosorbent assay (ELISA), T lymphocyte subsets were detected by flow cytometry, semiquantitative indexes of hepatitis B antigen / antibody were detected by time-resolved fluorescence analyzer, Biochemical detection of liver function. Results: (1) With the aggravation of liver inflammation, the content of XCL1 in serum gradually increased. The concentration of serum XCL1 in HBeAg + group was significantly higher than that in HBeAg + group (P <0.05). The serum concentration of IFN-γ in HBeAg- group was significantly higher than that in HBeAg + group (P <0.05). (2) The concentration of serum XCL1 was negatively correlated with the level of CD4 + T cells (r = -0.336, P = 0.017). The concentration of IFN-γ was positively correlated with the level of CD8 + T cells (r = 0.319, P = 0.024). Conclusion: Interferon gamma promotes the formation and development of chronic hepatitis B by affecting the expression of XCL1, and XCL1 is involved in the chronicity of hepatitis B.