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黄蜀葵花中富含黄酮类成分,具有保护心血管、改善肾功能等作用。该文采用3T3-L1前脂肪细胞诱导分化的成熟脂肪细胞,建立胰岛素抵抗模型,分为正常组,模型组,异槲皮苷(HY)、金丝桃苷(JY)、槲皮素(QT)、槲皮素-3’-O-葡萄糖苷(QG)、棉皮素-8-O-β-葡萄糖醛酸(GG)给药组,BCA法检测细胞培养液中葡萄糖的含量,荧光定量q PCR检测PPARγ,C/EBPα,SREBP-1,脂联素,内酯素,抵抗素基因mRNA的表达水平。结果显示,5种黄酮类化合物5μmol·L-1浓度时可促进3T3-L1前脂肪细胞的增殖,浓度为100μmol·L-1时可抑制前脂肪细胞的增殖;与正常组比较,模型组细胞对葡萄糖摄取量降低(P<0.01);与模型组相比,5种黄酮类化合物100μmol·L-1给药组细胞葡萄糖消耗量显著上升(P<0.01),PPARγ,C/EBPα,脂联素表达明显增加(P<0.01),SREBP-1,抵抗素,内酯素的表达明显降低(P<0.01),促使脂肪细胞分化。研究表明,HY,JY,QT,QG,GG能调控前脂肪细胞增殖,促进脂肪细胞分化及糖脂代谢相关因子PPARγ,C/EBPα,SREBP-1,脂联素,内酯素,抵抗素的表达,促进前脂肪细胞分化,增加葡萄糖利用,从而改善胰岛素抵抗。
Abelmoschus flowers rich in flavonoids, with the protection of cardiovascular, improve renal function and so on. The model of insulin resistance was established by using mature adipocytes induced by 3T3-L1 preadipocytes and divided into normal group, model group, isoquercitrin (HY), hyperoside (JY), quercetin (QT ), Quercetin-3’-O-glucoside (QG), and naringin-8-O-glucuronic acid (GG) administration group. BCA method was used to detect the glucose content in cell culture medium. q PCR was used to detect the mRNA expression of PPARγ, C / EBPα, SREBP-1, adiponectin, lactone and resistin. The results showed that 5 kinds of flavonoids can promote the proliferation of 3T3-L1 preadipocytes at the concentration of 5μmol·L-1, and inhibit the proliferation of preadipocytes at the concentration of 100μmol·L-1. Compared with the normal group, (P <0.01). Compared with the model group, the glucose consumption of 5 flavonoids in 100 μmol·L-1 administration group increased significantly (P <0.01), while PPARγ, C / EBPα, (P <0.01). The expressions of SREBP-1, resistin and lactone were significantly decreased (P <0.01), which induced adipocyte differentiation. Studies have shown that HY, JY, QT, QG, GG can regulate the proliferation of preadipocytes and promote adipocyte differentiation and related factors of glucose and lipid metabolism PPARγ, C / EBPα, SREBP-1, adiponectin, lactone, resistin Expression, promote preadipocyte differentiation, increase glucose utilization, thereby improving insulin resistance.