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1996年11月8日至13日在芝加哥召开了第47届美国肝病学会年会,现着重叙述庚型肝炎的研究进展。根椐从美国病人分离到的病毒株分析,HGV 为正链 RNA病毒,其基因组长度约为9 400个核苷酸,具有单一开放读码框架,可翻译成一长度为3 000个氨基酸的多蛋白(Polyprotein),在病毒本身及宿主细胞蛋白酶的作用下水解为具有各种功能的结构及非结构蛋白。其基因组织结构与黄病毒科的其它病毒(如 HCV)相似,具有5′UTR、结构区(E1,E2)和非结构区(NS1-NS5)及3′UTR。HGV RNA 与 HCV RNA 的核苷酸同源性小于30%,对基因表达调控起重要作用的5′UTR 和3′UTR 的初级序列与其它病毒均不相同,说明 HGV 是一个新的病毒。而从西非肝炎病人血清中分离出来的所谓 GBV-C 病毒的基因同源性与 HGV 相似,故二者被认为是同一病毒的不同分离株。因为5′UTR 的核苷酸序列最为保守,所以绝大数研究
The 47th annual meeting of the American College of Liver Diseases was held in Chicago from November 8 to November 13, 1996, highlighting the progress of research on hepatitis G. According to the analysis of virus strains isolated from American patients, HGV is a positive-stranded RNA virus with a genome length of about 9 400 nucleotides and a single open reading frame that can be translated into a polyprotein with a length of 3 000 amino acids (Polyprotein), in the virus itself and the host cell protease hydrolyzed into a variety of structural and non-structural proteins. Its genome structure is similar to other viruses of the Flaviviridae, such as HCV, and has 5 ’UTR, structural region (E1, E2) and non-structural region (NS1-NS5) and 3’ UTR. HGV RNA has less than 30% nucleotide homology with HCV RNA. The primary sequences of 5’UTR and 3’UTR that play an important role in gene expression regulation are different from other viruses, indicating that HGV is a new virus. However, the so-called GBV-C virus isolated from the serum of hepatitis B patients in Western Africa has similar genetic homology to HGV, so both are considered to be different isolates of the same virus. Because the 5 ’UTR nucleotide sequence is the most conservative, so the vast majority of studies