在人胚胎干细胞(human embryonic stem,hES)的研究中,有效的冻存方法非常重要。目前,冷冻ES细胞主要有两种方法:慢速冷冻-快速复温和超快速玻璃化法。慢冻法需要昂贵的程序性冷冻仪,而玻璃化法步骤繁琐且只能冻存小量细胞,不能满足临床应用。因此,本研究旨在寻找非程序性冷冻仪 In the research of human embryonic stem cells (hES), the effective cryopreservation method is very important. There are currently two main ways to freeze ES cells: slow freezing - rapid rewarming and ultra-fast vitrification. Slow-freezing method requires expensive procedural freezer, and vitrification steps cumbersome and can only freeze a small amount of cells, can not meet the clinical application. Therefore, this study aims to find a non-procedural freezer