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目的探讨赣江水体有机提取物的雌激素活性及遗传毒性,从而为赣江水体污染的环境风险评价提供科学的理论依据。方法分别从赣江的上游、中游和下游各3个断面的水体采集水样,接着采用XAD-2大孔树脂对水样中的有机物进行浓缩提取,然后利用子宫增重实验检测有机提取物的雌激素活性,并利用微核实验检测有机提取物的遗传毒性。结果各水样有机提取物均显示出雌激素活性和遗传毒性,且具有剂量-反应关系,与溶剂对照组比较,差异具有统计学意义(P<0.01);不同采样断面水样有机提取物的雌激素活性有所不同,总体趋势是下游>中游>上游;下游水样有机提取物所致微核率明显地高于上游和中游水样(P<0.01);而上游水样有机提取物所致的微核率与中游之间的差异则无统计学意义(P>0.05)。结论赣江水体已受到环境内分泌干扰物的污染并具有一定的遗传毒性,对环境及人群健康具有潜在的风险。
Objective To investigate the estrogenic activity and genotoxicity of organic extracts of Ganjiang River water, so as to provide a scientific theoretical basis for environmental risk assessment of water pollution in Ganjiang River. Methods Water samples were collected from three sections of the upper, middle and downstream sections of Ganjiang River respectively. Then the organic compounds in the water sample were concentrated and extracted by XAD-2 macroporous resin. Then the contents of organic extracts Estrogen activity, and the micronucleus test was used to determine the genotoxicity of the organic extract. Results The organic extracts of all samples showed estrogenic activity and genotoxicity with a dose-response relationship. Compared with the solvent control group, the differences were statistically significant (P <0.01). The organic extracts of different sampling sections Estrogen activity is different, the overall trend is downstream> midstream> upstream; micronucleus rate of organic extracts from downstream water samples is significantly higher than upstream and midstream water samples (P <0.01); while upstream water samples organic extract The difference between micronucleus rate and middle reaches was not statistically significant (P> 0.05). Conclusion The Ganjiang River water body has been contaminated by environmental endocrine disruptors and has certain genotoxicity, which poses potential risks to the environment and population health.