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目的探讨血管内皮生长因子(-460C/T,-1154G/A,-2578C/A,+936C/T)基因多态性与异位妊娠的发病风险的关系。方法采用病例-对照研究方法,以192例异位妊娠患者和210例健康绝经后女性(至少2次分娩史的健康女性,均无异位妊娠流产病史)为研究对象,采用聚合酶链反应-限制性片段长度多态性(polymerase chain reactionrestriction fragment length polymorphism,PCR-RFLP)方法对血管内皮生长因子-460C/T,-1154G/A,-2578C/A,+936C/T这4个SNPs位点进行基因分型。结果 VEGF-460 C/T和+936C/T基因型及等位基因频率分布在病例和对照组无显著差异(P>0.05)。与G/G基因型相比,携带VEGF-1154G/A的A等位基因型(G/A+A/A基因型)可显著降低异位妊娠的发病风险(OR=0.61,95%CI=0.42-0.87)。与C/C基因型相比,携带VEGF-2578C/A的A等位基因型(A/A+C/A基因型)可显著降低异位妊娠的发病风险(OR=0.66,95%CI=0.44-0.99)。与TGC单体型相比,TAA和CAA单体型均可明显降低异位妊娠的发病风险(P=0.020,OR=0.41,95%CI=0.19-0.89;P=0.014,OR=0.29,95%CI=0.11-0.82)。结论携带VEGF-1154G/A和-2578C/A多态的A等位基因型可能显著降低异位妊娠的发病风险,提示该等位基因可能是异位妊娠发病的一个潜在的保护因素;VEGF-460C/T、-1154G/A和-2578C/A三个多态位点间存在部分连锁不平衡现象,TAA和CAA单体型均可明显降低异位妊娠的发病风险。
Objective To investigate the relationship between the polymorphisms of vascular endothelial growth factor (-460C / T, -1154G / A, -2578C / A, +936C / T) and the risk of ectopic pregnancy. Methods A case-control study was conducted in 192 ectopic pregnancy patients and 210 healthy post-menopausal women (healthy women with at least 2 childbirth history, no history of ectopic pregnancy miscarriage). The patients were divided into two groups by polymerase chain reaction- The four SNPs of vascular endothelial growth factor-460C / T, -1154G / A, -2578C / A and + 936C / T were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) Genotyping. Results The frequencies of genotypes and alleles of VEGF-460 C / T and + 936C / T were not significantly different between cases and controls (P> 0.05). The A allele (G / A + A / A genotype) carrying VEGF-1154G / A significantly reduced the risk of ectopic pregnancy compared with G / G genotype (OR = 0.61, 95% CI = 0.42-0.87). The A allele (A / A + C / A genotype) carrying VEGF-2578C / A significantly reduced the risk of ectopic pregnancy compared with C / C genotype (OR = 0.66, 95% CI = 0.44-0.99). Compared with TGC haplotype, both TAA and CAA haplotypes significantly reduced the risk of ectopic pregnancy (P = 0.020, OR = 0.41, 95% CI = 0.19-0.89; P = 0.014, OR = 0.29, 95 % CI = 0.11-0.82). Conclusion A allele carrying VEGF-1154G / A and -2578C / A polymorphisms may significantly reduce the risk of ectopic pregnancy, suggesting that the allele might be a potential protective factor in the pathogenesis of ectopic pregnancy. VEGF- 460C / T, -1154G / A and -2578C / A three polymorphic sites exist partial linkage disequilibrium, TAA and CAA haplotype can significantly reduce the risk of ectopic pregnancy.