论文部分内容阅读
对中国主要淡水湖泊(鄱阳湖、洞庭湖、太湖、洪泽湖及巢湖)三角帆蚌5个野生群体的线粒体DNA 16S rRNA基因片段进行了扩增和测序,得到473bp的碱基序列,没有发现插入/缺失突变的核苷酸位点。检测到了32个多态性核苷酸位点,共7种单倍型。鄱阳湖群体的222(C→G)和325(A→G)位点,太湖群体的233(A→G)位点,巢湖群体的40(A→G)、138(A→T)和294(C→T)位点,洪泽湖群体的241(A→C)位点的变异可以作为区分群体分子遗传标记位点。洞庭湖群体未发现特异位点。在5个群体间鄱阳湖群体多态性位点、核苷酸多态性、单倍型多态性和单倍型数量4个指标都最高,表明鄱阳湖群体具有最为丰富的遗传结构,遗传变异最大,可作为三角帆蚌选育的基础群体。
The mitochondrial DNA 16S rRNA gene fragment was amplified and sequenced from 5 wild populations of Hyriopsis cumingii in China’s major freshwater lakes (Poyang Lake, Dongting Lake, Taihu Lake, Hongze Lake and Chaohu Lake), and a 473bp base sequence was obtained. Insert / delete mutated nucleotide sites. A total of 32 haplotypes were detected in 32 polymorphic nucleotide sites. (P → G) and 325 (A → G) sites in Poyang Lake, 233 (A → G) sites in Taihu Lake, 40 (A → G), 138 (A → T) and 294 (C → T) locus, the variation of 241 (A → C) locus in Hongzehu population can be used as a marker to distinguish groups of molecular genetic markers. Dongting Lake population found no specific sites. Poyang Lake population polymorphism loci, nucleotide polymorphism, haplotype polymorphism and haplotype number were the highest among the five populations, indicating that Poyang Lake population has the most abundant genetic structure, genetic Variation of the largest, can be used as the basic group breeding Hyriopsis cumingii.