论文部分内容阅读
目的了解2008年珠海市Victoria系B型流感病毒的HA1基因特性。方法采集珠海市流感样病例咽拭子样本进行流感病毒的分离和鉴定,并对分离到的B型流感病毒进行核酸的提取,采用逆转录-聚合酶链反应(RT-PCR)扩增病毒基因后进行病毒血凝素HA1基因核苷酸序列测定,用Mega、DNAStar、GeneDoc软件对测序结果进行分析。结果 2008年分离到的4株Victoria系B型流感毒株通过基因进化树分析均在1个分支上,与同时段的疫苗株B/Malaysia/2506/2004的核苷酸同源性高达98.6%～99.1%。通过氨基酸分析发现,在HA1基因的第70～90位区域未发现氨基酸变异,而在110～210区域,均在134号位点发生丝氨酸到脯氨酸的替换,在165位有个别株出现了天冬酰胺到赖氨酸的替换。与B/Malaysia/2506/2004和B/Shenzhen/155/05比较,在199位出现了苏氨酸的替换,使毒株在197～199位上的氨基酸形成Asn-Glu-Thr的结果,导致在该处增加了1个潜在的糖基化位点。结论 2008年珠海市人群中流行的Victoria系B型流感病毒与疫苗株B/Malaysia/2506/2004相比抗原性已经发生了变化。
Objective To understand the characteristics of HA1 gene of influenza B virus strains in Zhuhai City in 2008. Methods Throat swab specimens from influenza-like cases in Zhuhai were collected for influenza virus isolation and identification. Nucleic acid was isolated from influenza B virus. The viral genes were amplified by reverse transcription-polymerase chain reaction (RT-PCR) After the virus hemagglutinin HA1 gene nucleotide sequence determination, using Mega, DNAStar, GeneDoc software sequencing results were analyzed. Results Four isolates of type B virus from Victoria in 2008 were all found in one branch by phylogenetic tree analysis. The homology of nucleotide sequence with the vaccine strain B / Malaysia / 2506/2004 was up to 98.6% ~ 99.1%. Amino acid analysis showed no amino acid variation was found in the 70th to 90th regions of HA1 gene, while serine to proline substitution occurred in the region of 110 to 210 at position 134, and individual strains appeared at 165 Asparagine to Lysine Replacement. Compared with B / Malaysia / 2506/2004 and B / Shenzhen / 155/05, a threonine substitution at position 199 led to the formation of Asn-Glu-Thr by the amino acids at position 197-199 of the strain, resulting in There is an additional potential glycosylation site added. Conclusion The prevalence of influenza B virus in Zhuhai population in 2008 has changed compared with vaccine strain B / Malaysia / 2506/2004.